温敏型蛋白质分子印迹聚合物的制备及性能研究

Xiang Zhi Dong; Yong Ma; Chun Ping Hou; Bao Liang Zhang; He Peng Zhang; Qiu Yu Zhang

doi:10.16606/j.cnki.issn0253-4320.2019.10.019

温敏型蛋白质分子印迹聚合物的制备及性能研究

Translated title of the contribution: Preparation and properties of thermo-sensitive protein molecularly imprinted polymers

Xiang Zhi Dong, Yong Ma, Chun Ping Hou, Bao Liang Zhang, He Peng Zhang, Qiu Yu Zhang

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Using modified SiO₂ nanoparticles as carrier, acrylamide (AAM) and N-isopropyl acrylamide (NIPAM) as functional monomers, N, N'-methylene diacrylamide (MBA) as cross-linking agent and bovine serum albumin (BSA) as a template protein, a highly selective protein imprinting polymer is prepared. The results from IR and transmission electron microscope (TEM) demonstrate that the imprinting layer is successfully grafted onto the surface of the carrier.Under the optimum experimental conditions, the adsorption capacities of the imprinted polymer (MIP) and the non-imprinted polymer (NIP) are 67.445 mg•g^-1 and 38.248 mg•g^-1, respectively, and the selectivity factor is 1.763. Three experimental schemes are designed to evaluate the selectivity of MIP and the results show MIP has a good specific recognition for template protein BSA, which provides a new method for protein identification.

Translated title of the contribution	Preparation and properties of thermo-sensitive protein molecularly imprinted polymers
Original language	Chinese (Traditional)
Pages (from-to)	86-91
Number of pages	6
Journal	Xiandai Huagong/Modern Chemical Industry
Volume	39
Issue number	10
DOIs	https://doi.org/10.16606/j.cnki.issn0253-4320.2019.10.019
State	Published - 20 Oct 2019

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title = "温敏型蛋白质分子印迹聚合物的制备及性能研究",

abstract = "Using modified SiO2 nanoparticles as carrier, acrylamide (AAM) and N-isopropyl acrylamide (NIPAM) as functional monomers, N, N'-methylene diacrylamide (MBA) as cross-linking agent and bovine serum albumin (BSA) as a template protein, a highly selective protein imprinting polymer is prepared. The results from IR and transmission electron microscope (TEM) demonstrate that the imprinting layer is successfully grafted onto the surface of the carrier.Under the optimum experimental conditions, the adsorption capacities of the imprinted polymer (MIP) and the non-imprinted polymer (NIP) are 67.445 mg•g-1 and 38.248 mg•g-1, respectively, and the selectivity factor is 1.763. Three experimental schemes are designed to evaluate the selectivity of MIP and the results show MIP has a good specific recognition for template protein BSA, which provides a new method for protein identification.",

keywords = "Rebinding adsorption, Selectivity, Surface imprinting, Thermo-sensitivity",

author = "Dong, {Xiang Zhi} and Yong Ma and Hou, {Chun Ping} and Zhang, {Bao Liang} and Zhang, {He Peng} and Zhang, {Qiu Yu}",

year = "2019",

month = oct,

day = "20",

doi = "10.16606/j.cnki.issn0253-4320.2019.10.019",

language = "繁体中文",

volume = "39",

pages = "86--91",

journal = "Xiandai Huagong/Modern Chemical Industry",

issn = "0253-4320",

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T1 - 温敏型蛋白质分子印迹聚合物的制备及性能研究

AU - Dong, Xiang Zhi

AU - Ma, Yong

AU - Hou, Chun Ping

AU - Zhang, Bao Liang

AU - Zhang, He Peng

AU - Zhang, Qiu Yu

PY - 2019/10/20

Y1 - 2019/10/20

N2 - Using modified SiO2 nanoparticles as carrier, acrylamide (AAM) and N-isopropyl acrylamide (NIPAM) as functional monomers, N, N'-methylene diacrylamide (MBA) as cross-linking agent and bovine serum albumin (BSA) as a template protein, a highly selective protein imprinting polymer is prepared. The results from IR and transmission electron microscope (TEM) demonstrate that the imprinting layer is successfully grafted onto the surface of the carrier.Under the optimum experimental conditions, the adsorption capacities of the imprinted polymer (MIP) and the non-imprinted polymer (NIP) are 67.445 mg•g-1 and 38.248 mg•g-1, respectively, and the selectivity factor is 1.763. Three experimental schemes are designed to evaluate the selectivity of MIP and the results show MIP has a good specific recognition for template protein BSA, which provides a new method for protein identification.

AB - Using modified SiO2 nanoparticles as carrier, acrylamide (AAM) and N-isopropyl acrylamide (NIPAM) as functional monomers, N, N'-methylene diacrylamide (MBA) as cross-linking agent and bovine serum albumin (BSA) as a template protein, a highly selective protein imprinting polymer is prepared. The results from IR and transmission electron microscope (TEM) demonstrate that the imprinting layer is successfully grafted onto the surface of the carrier.Under the optimum experimental conditions, the adsorption capacities of the imprinted polymer (MIP) and the non-imprinted polymer (NIP) are 67.445 mg•g-1 and 38.248 mg•g-1, respectively, and the selectivity factor is 1.763. Three experimental schemes are designed to evaluate the selectivity of MIP and the results show MIP has a good specific recognition for template protein BSA, which provides a new method for protein identification.

KW - Rebinding adsorption

KW - Selectivity

KW - Surface imprinting

KW - Thermo-sensitivity

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U2 - 10.16606/j.cnki.issn0253-4320.2019.10.019

DO - 10.16606/j.cnki.issn0253-4320.2019.10.019

M3 - 文章

AN - SCOPUS:85074793450

SN - 0253-4320

VL - 39

SP - 86

EP - 91

JO - Xiandai Huagong/Modern Chemical Industry

JF - Xiandai Huagong/Modern Chemical Industry

IS - 10

ER -

温敏型蛋白质分子印迹聚合物的制备及性能研究

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