Rational Design of a Two-Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues

Haixiao Fang; Hang Zhang; Lin Li; Yun Ni; Riri Shi; Zheng Li; Xuekang Yang; Bo Ma; Chengwu Zhang; Qiong Wu; Changmin Yu; Naidi Yang; Shao Q. Yao; Wei Huang

doi:10.1002/anie.202000059

Rational Design of a Two-Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues

Haixiao Fang, Hang Zhang, Lin Li, Yun Ni, Riri Shi, Zheng Li, Xuekang Yang, Bo Ma, Chengwu Zhang, Qiong Wu, Changmin Yu, Naidi Yang, Shao Q. Yao, Wei Huang

柔性电子研究院

科研成果: 期刊稿件 › 文章 › 同行评审

67 引用（Scopus）

摘要

Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO-A and MAO-B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A-expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.

源语言	英语
页（从-至）	7536-7541
页数	6
期刊	Angewandte Chemie - International Edition
卷	59
期	19
DOI	https://doi.org/10.1002/anie.202000059
出版状态	已出版 - 4 5月 2020

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abstract = "Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO-A and MAO-B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A-expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.",

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AU - Fang, Haixiao

AU - Zhang, Hang

AU - Li, Lin

AU - Ni, Yun

AU - Shi, Riri

AU - Li, Zheng

AU - Yang, Xuekang

AU - Ma, Bo

AU - Zhang, Chengwu

AU - Wu, Qiong

AU - Yu, Changmin

AU - Yang, Naidi

AU - Yao, Shao Q.

AU - Huang, Wei

PY - 2020/5/4

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N2 - Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO-A and MAO-B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A-expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.

AB - Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO-A and MAO-B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A-expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.

KW - bioimaging

KW - fluorogenic probes

KW - glioma

KW - monoamine oxidase A

KW - two-photon fluorescence microscopy

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Rational Design of a Two-Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues

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