Investigation on osteoblast growth on the modified surface of porous titanium

Three methods have been used to modify the porous titanium surface, which is the alkali heat treatment, alkali heat treatment + SBF solution soak and alkali heat treatment + precalcidied + SBF solution soak. The morphology of different surfaces was observed by scanning electron microscopy (SEM). The MC3T3-E1 osteoblast cell was cultured on the modified and unmodified surface of porous titanium with 3days and 7days, the morphology of osteoblast adhesion and growth on different surface was observed. The results showed that osteoblast adhere on the modified and unmodified surface of the porous titanium. Osteoblast on AHS and HA modified surface can grow and spread, but it cannot grow and spread on unmodified and AH modified surface of the porous titanium. Osteoblast can grow across the different titanium fibers of on HA modified surface of porous titanium. Osteoblast on the HA surface has the very good biological suitability, which is beneficial to the combination of bone tissue and porous titanium.