Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition

A. Wei; X. W. Sun; J. X. Wang; Y. Lei; X. P. Cai; C. M. Li; Z. L. Dong; W. Huang

doi:10.1063/1.2356307

Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition

A. Wei, X. W. Sun, J. X. Wang, Y. Lei, X. P. Cai, C. M. Li, Z. L. Dong, W. Huang

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We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm^-2 mM^-1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods.

源语言	英语
文章编号	123902
期刊	Applied Physics Letters
卷	89
期	12
DOI	https://doi.org/10.1063/1.2356307
出版状态	已出版 - 2006
已对外发布	是

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abstract = "We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm-2 mM-1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods.",

author = "A. Wei and Sun, {X. W.} and Wang, {J. X.} and Y. Lei and Cai, {X. P.} and Li, {C. M.} and Dong, {Z. L.} and W. Huang",

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AU - Wei, A.

AU - Sun, X. W.

AU - Wang, J. X.

AU - Lei, Y.

AU - Cai, X. P.

AU - Li, C. M.

AU - Dong, Z. L.

AU - Huang, W.

PY - 2006

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N2 - We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm-2 mM-1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods.

AB - We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm-2 mM-1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods.

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JO - Applied Physics Letters

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Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition

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