Abstract
The aim of this study was to investigate the effects of the clinostat-simulated weightlessness on biological characteristics of MLO-Y4 osteocyte-like cells. MLO-Y4 cells were incubated for 24 h, then randomly divided into 3 groups and rotated in a clinostat as a model of simulated weightlessness for 12 h, 24 h and 48 h. The morphology, cytoskeleton, and secretion of soluble molecules of MLO-Y4 cells were observed and detected. The results show that clinostat culture affects the number of dendrites/cell, cytoskeleton distribution, and secretion of nitric oxide and prostaglandin E2 in MLO-Y4 cells. These results may provide some clue to explore the cellular mechanism of bone loss caused by weightlessness.
| Original language | English |
|---|---|
| Pages (from-to) | 255-262 |
| Number of pages | 8 |
| Journal | Biologia |
| Volume | 67 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2012 |
Keywords
- 2D clinostat
- bone loss
- cellular morphology
- cytoskeleton
- osteocyte
- secretion
- weightlessness