Strong nonlinear optical phosphorescence from water-soluble polymer dots: Towards the application of two-photon bioimaging

Tingchao He; Wenbo Hu; Huifang Shi; Qunfeng Pan; Guohong Ma; Wei Huang; Quli Fan; Xiaodong Lin

doi:10.1016/j.dyepig.2015.08.002

Strong nonlinear optical phosphorescence from water-soluble polymer dots: Towards the application of two-photon bioimaging

Tingchao He, Wenbo Hu, Huifang Shi, Qunfeng Pan, Guohong Ma, Wei Huang, Quli Fan, Xiaodong Lin

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Multiphoton fluorescence imaging of tissues offers an essential tool for studying biological systems. However, autofluorescence becomes a serious issue in complicated tissue imaging where biological molecules compete with the fluorophore. Such a critical issue can be resolved through the design of phosphorescent molecules with long emission lifetime. In this work, the nonlinear optical properties of small phosphorescent polymer dots (Pdots) with strong red-emitting phosphorescence (620 nm) have been disclosed. An analysis of decay data from fs pump-probe experiment yields a value of 164 ps for the singlet excited state lifetime of the Pdots. Notably, thanks to efficient two-photon excited energy transfer and moderate phosphorescence quantum yield (η = 1.36%), the Pdots exhibit large two-photon absorption action cross-section (ηδ_max = 15 GM) in aqueous solution. Results of living cell imaging experiments show the potential of these Pdots in two-photon time-resolved phosphorescent microscopy.

Original language	English
Pages (from-to)	218-221
Number of pages	4
Journal	Dyes and Pigments
Volume	123
DOIs	https://doi.org/10.1016/j.dyepig.2015.08.002
State	Published - 26 Aug 2015
Externally published	Yes

Keywords

Bioimaging
FRET
Polymer dots
Two-photon absorption
Two-photon excited phosphorescence
Z-scan measurement

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10.1016/j.dyepig.2015.08.002

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title = "Strong nonlinear optical phosphorescence from water-soluble polymer dots: Towards the application of two-photon bioimaging",

abstract = "Multiphoton fluorescence imaging of tissues offers an essential tool for studying biological systems. However, autofluorescence becomes a serious issue in complicated tissue imaging where biological molecules compete with the fluorophore. Such a critical issue can be resolved through the design of phosphorescent molecules with long emission lifetime. In this work, the nonlinear optical properties of small phosphorescent polymer dots (Pdots) with strong red-emitting phosphorescence (620 nm) have been disclosed. An analysis of decay data from fs pump-probe experiment yields a value of 164 ps for the singlet excited state lifetime of the Pdots. Notably, thanks to efficient two-photon excited energy transfer and moderate phosphorescence quantum yield (η = 1.36%), the Pdots exhibit large two-photon absorption action cross-section (ηδmax = 15 GM) in aqueous solution. Results of living cell imaging experiments show the potential of these Pdots in two-photon time-resolved phosphorescent microscopy.",

keywords = "Bioimaging, FRET, Polymer dots, Two-photon absorption, Two-photon excited phosphorescence, Z-scan measurement",

author = "Tingchao He and Wenbo Hu and Huifang Shi and Qunfeng Pan and Guohong Ma and Wei Huang and Quli Fan and Xiaodong Lin",

year = "2015",

month = aug,

day = "26",

doi = "10.1016/j.dyepig.2015.08.002",

language = "英语",

volume = "123",

pages = "218--221",

journal = "Dyes and Pigments",

issn = "0143-7208",

publisher = "Elsevier B.V.",

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T1 - Strong nonlinear optical phosphorescence from water-soluble polymer dots

T2 - Towards the application of two-photon bioimaging

AU - He, Tingchao

AU - Hu, Wenbo

AU - Shi, Huifang

AU - Pan, Qunfeng

AU - Ma, Guohong

AU - Huang, Wei

AU - Fan, Quli

AU - Lin, Xiaodong

PY - 2015/8/26

Y1 - 2015/8/26

N2 - Multiphoton fluorescence imaging of tissues offers an essential tool for studying biological systems. However, autofluorescence becomes a serious issue in complicated tissue imaging where biological molecules compete with the fluorophore. Such a critical issue can be resolved through the design of phosphorescent molecules with long emission lifetime. In this work, the nonlinear optical properties of small phosphorescent polymer dots (Pdots) with strong red-emitting phosphorescence (620 nm) have been disclosed. An analysis of decay data from fs pump-probe experiment yields a value of 164 ps for the singlet excited state lifetime of the Pdots. Notably, thanks to efficient two-photon excited energy transfer and moderate phosphorescence quantum yield (η = 1.36%), the Pdots exhibit large two-photon absorption action cross-section (ηδmax = 15 GM) in aqueous solution. Results of living cell imaging experiments show the potential of these Pdots in two-photon time-resolved phosphorescent microscopy.

AB - Multiphoton fluorescence imaging of tissues offers an essential tool for studying biological systems. However, autofluorescence becomes a serious issue in complicated tissue imaging where biological molecules compete with the fluorophore. Such a critical issue can be resolved through the design of phosphorescent molecules with long emission lifetime. In this work, the nonlinear optical properties of small phosphorescent polymer dots (Pdots) with strong red-emitting phosphorescence (620 nm) have been disclosed. An analysis of decay data from fs pump-probe experiment yields a value of 164 ps for the singlet excited state lifetime of the Pdots. Notably, thanks to efficient two-photon excited energy transfer and moderate phosphorescence quantum yield (η = 1.36%), the Pdots exhibit large two-photon absorption action cross-section (ηδmax = 15 GM) in aqueous solution. Results of living cell imaging experiments show the potential of these Pdots in two-photon time-resolved phosphorescent microscopy.

KW - Bioimaging

KW - FRET

KW - Polymer dots

KW - Two-photon absorption

KW - Two-photon excited phosphorescence

KW - Z-scan measurement

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U2 - 10.1016/j.dyepig.2015.08.002

DO - 10.1016/j.dyepig.2015.08.002

M3 - 文章

AN - SCOPUS:84940180954

SN - 0143-7208

VL - 123

SP - 218

EP - 221

JO - Dyes and Pigments

JF - Dyes and Pigments

ER -

Strong nonlinear optical phosphorescence from water-soluble polymer dots: Towards the application of two-photon bioimaging

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Keywords

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