Simulated microgravity alters the expression of cytoskeleton- and ATP-binding-related genes in MLO-Y4 osteocytes

Zhihao Chen; Fan Zhao; Yiduo Qi; Lifang Hu; Dijie Li; Chong Yin; Peihong Su; Yan Zhang; Jianhua Ma; Jing Qian; Hongpo Zhou; Yiwei Zou; Airong Qian

doi:10.1016/j.actaastro.2016.09.017

Simulated microgravity alters the expression of cytoskeleton- and ATP-binding-related genes in MLO-Y4 osteocytes

Zhihao Chen, Fan Zhao, Yiduo Qi, Lifang Hu, Dijie Li, Chong Yin, Peihong Su, Yan Zhang, Jianhua Ma, Jing Qian, Hongpo Zhou, Yiwei Zou, Airong Qian

School of life sciences

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Bone undergoes dynamic modelling and remodelling processes, and it requires gravity-mediated mechanical stimulation for the maintenance of mineral content and structure. Osteocytes are the most commonly found cells in the mature bone, and they are sensitive to mechanical changes. The purpose of this study was to investigate the effects of microgravity simulated with a random position machine (RPM) on the gene expression profile of osteocytes. Genes sensitive to RPM treatment were sorted on the basis of biological processes, interactions and signalling pathways. Overall, 504 differentially expressed genes (DEGs) in osteocytes cultured under RPM conditions were found. The DEGs were further analysed using bioinformatics tools such as DAVID and iReport. A total of 15 ATP-binding and cytoskeleton-related genes were further confirmed by quantitative real-time PCR (qRT-PCR). Our findings demonstrate that the RPM affected the expression of genes involved in cytoskeleton remodelling and the energy-transfer process in osteocytes. The identification of mechanosensitive genes may enhance our understanding of the roles of osteocytes in mechanosensation and may provide some potential targets for preventing and treating bone-related diseases.

Original language	English
Pages (from-to)	186-192
Number of pages	7
Journal	Acta Astronautica
Volume	129
DOIs	https://doi.org/10.1016/j.actaastro.2016.09.017
State	Published - 1 Dec 2016

Keywords

ATP-binding
Cytoskeleton
Gene expression profile
Osteocyte
Random position machine (RPM)
Simulated microgravity

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10.1016/j.actaastro.2016.09.017

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title = "Simulated microgravity alters the expression of cytoskeleton- and ATP-binding-related genes in MLO-Y4 osteocytes",

abstract = "Bone undergoes dynamic modelling and remodelling processes, and it requires gravity-mediated mechanical stimulation for the maintenance of mineral content and structure. Osteocytes are the most commonly found cells in the mature bone, and they are sensitive to mechanical changes. The purpose of this study was to investigate the effects of microgravity simulated with a random position machine (RPM) on the gene expression profile of osteocytes. Genes sensitive to RPM treatment were sorted on the basis of biological processes, interactions and signalling pathways. Overall, 504 differentially expressed genes (DEGs) in osteocytes cultured under RPM conditions were found. The DEGs were further analysed using bioinformatics tools such as DAVID and iReport. A total of 15 ATP-binding and cytoskeleton-related genes were further confirmed by quantitative real-time PCR (qRT-PCR). Our findings demonstrate that the RPM affected the expression of genes involved in cytoskeleton remodelling and the energy-transfer process in osteocytes. The identification of mechanosensitive genes may enhance our understanding of the roles of osteocytes in mechanosensation and may provide some potential targets for preventing and treating bone-related diseases.",

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author = "Zhihao Chen and Fan Zhao and Yiduo Qi and Lifang Hu and Dijie Li and Chong Yin and Peihong Su and Yan Zhang and Jianhua Ma and Jing Qian and Hongpo Zhou and Yiwei Zou and Airong Qian",

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doi = "10.1016/j.actaastro.2016.09.017",

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T1 - Simulated microgravity alters the expression of cytoskeleton- and ATP-binding-related genes in MLO-Y4 osteocytes

AU - Chen, Zhihao

AU - Zhao, Fan

AU - Qi, Yiduo

AU - Hu, Lifang

AU - Li, Dijie

AU - Yin, Chong

AU - Su, Peihong

AU - Zhang, Yan

AU - Ma, Jianhua

AU - Qian, Jing

AU - Zhou, Hongpo

AU - Zou, Yiwei

AU - Qian, Airong

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Bone undergoes dynamic modelling and remodelling processes, and it requires gravity-mediated mechanical stimulation for the maintenance of mineral content and structure. Osteocytes are the most commonly found cells in the mature bone, and they are sensitive to mechanical changes. The purpose of this study was to investigate the effects of microgravity simulated with a random position machine (RPM) on the gene expression profile of osteocytes. Genes sensitive to RPM treatment were sorted on the basis of biological processes, interactions and signalling pathways. Overall, 504 differentially expressed genes (DEGs) in osteocytes cultured under RPM conditions were found. The DEGs were further analysed using bioinformatics tools such as DAVID and iReport. A total of 15 ATP-binding and cytoskeleton-related genes were further confirmed by quantitative real-time PCR (qRT-PCR). Our findings demonstrate that the RPM affected the expression of genes involved in cytoskeleton remodelling and the energy-transfer process in osteocytes. The identification of mechanosensitive genes may enhance our understanding of the roles of osteocytes in mechanosensation and may provide some potential targets for preventing and treating bone-related diseases.

AB - Bone undergoes dynamic modelling and remodelling processes, and it requires gravity-mediated mechanical stimulation for the maintenance of mineral content and structure. Osteocytes are the most commonly found cells in the mature bone, and they are sensitive to mechanical changes. The purpose of this study was to investigate the effects of microgravity simulated with a random position machine (RPM) on the gene expression profile of osteocytes. Genes sensitive to RPM treatment were sorted on the basis of biological processes, interactions and signalling pathways. Overall, 504 differentially expressed genes (DEGs) in osteocytes cultured under RPM conditions were found. The DEGs were further analysed using bioinformatics tools such as DAVID and iReport. A total of 15 ATP-binding and cytoskeleton-related genes were further confirmed by quantitative real-time PCR (qRT-PCR). Our findings demonstrate that the RPM affected the expression of genes involved in cytoskeleton remodelling and the energy-transfer process in osteocytes. The identification of mechanosensitive genes may enhance our understanding of the roles of osteocytes in mechanosensation and may provide some potential targets for preventing and treating bone-related diseases.

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KW - Cytoskeleton

KW - Gene expression profile

KW - Osteocyte

KW - Random position machine (RPM)

KW - Simulated microgravity

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Simulated microgravity alters the expression of cytoskeleton- and ATP-binding-related genes in MLO-Y4 osteocytes

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Keywords

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