Replacing a reservoir solution with desiccant in vapor diffusion protein crystallization screening

Qin Qin Lu; Da Chuan Yin; Rui Qing Chen; Si Xiao Xie; Yong Ming Liu; Xian Fang Zhang; Li Zhu; Zheng Tang Liu; Peng Shang

doi:10.1107/S0021889810030803

Replacing a reservoir solution with desiccant in vapor diffusion protein crystallization screening

Qin Qin Lu, Da Chuan Yin, Rui Qing Chen, Si Xiao Xie, Yong Ming Liu, Xian Fang Zhang, Li Zhu, Zheng Tang Liu, Peng Shang

School of life sciences

Northwestern Polytechnical University Xian

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

This paper presents a modification to the conventional vapor diffusion (hanging-or sitting-drop) technique for protein crystallization screening. In this modified method, the reservoir solution is replaced with a desiccant to allow for a larger range of protein solution concentrations, thereby providing more opportunities for crystal formation. This method was tested in both reproducibility and screening studies, and the results showed that it significantly improves the efficiency and reduces the cost of protein crystallization screens.

Original language	English
Pages (from-to)	1021-1026
Number of pages	6
Journal	Journal of Applied Crystallography
Volume	43
Issue number	5 PART 1
DOIs	https://doi.org/10.1107/S0021889810030803
State	Published - 2010

Keywords

crystallization screening
desiccants
protein crystallization
vapor diffusion

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10.1107/S0021889810030803

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abstract = "This paper presents a modification to the conventional vapor diffusion (hanging-or sitting-drop) technique for protein crystallization screening. In this modified method, the reservoir solution is replaced with a desiccant to allow for a larger range of protein solution concentrations, thereby providing more opportunities for crystal formation. This method was tested in both reproducibility and screening studies, and the results showed that it significantly improves the efficiency and reduces the cost of protein crystallization screens.",

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author = "Lu, {Qin Qin} and Yin, {Da Chuan} and Chen, {Rui Qing} and Xie, {Si Xiao} and Liu, {Yong Ming} and Zhang, {Xian Fang} and Li Zhu and Liu, {Zheng Tang} and Peng Shang",

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AU - Lu, Qin Qin

AU - Yin, Da Chuan

AU - Chen, Rui Qing

AU - Xie, Si Xiao

AU - Liu, Yong Ming

AU - Zhang, Xian Fang

AU - Zhu, Li

AU - Liu, Zheng Tang

AU - Shang, Peng

PY - 2010

Y1 - 2010

N2 - This paper presents a modification to the conventional vapor diffusion (hanging-or sitting-drop) technique for protein crystallization screening. In this modified method, the reservoir solution is replaced with a desiccant to allow for a larger range of protein solution concentrations, thereby providing more opportunities for crystal formation. This method was tested in both reproducibility and screening studies, and the results showed that it significantly improves the efficiency and reduces the cost of protein crystallization screens.

AB - This paper presents a modification to the conventional vapor diffusion (hanging-or sitting-drop) technique for protein crystallization screening. In this modified method, the reservoir solution is replaced with a desiccant to allow for a larger range of protein solution concentrations, thereby providing more opportunities for crystal formation. This method was tested in both reproducibility and screening studies, and the results showed that it significantly improves the efficiency and reduces the cost of protein crystallization screens.

KW - crystallization screening

KW - desiccants

KW - protein crystallization

KW - vapor diffusion

UR - http://www.scopus.com/inward/record.url?scp=77957373877&partnerID=8YFLogxK

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DO - 10.1107/S0021889810030803

M3 - 文章

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JO - Journal of Applied Crystallography

JF - Journal of Applied Crystallography

IS - 5 PART 1

ER -

Replacing a reservoir solution with desiccant in vapor diffusion protein crystallization screening

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Keywords

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