Quantitative phase microscopy for cellular dynamics based on transport of intensity equation

Ying Li, Jianglei Di, Chaojie Ma, Jiwei Zhang, Jinzhan Zhong, Kaiqiang Wang, Teli Xi, Jianlin Zhao

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

We demonstrate a simple method for quantitative phase imaging of tiny transparent objects such as living cells based on the transport of intensity equation. The experiments are performed using an inverted bright field microscope upgraded with a flipping imaging module, which enables to simultaneously create two laterally separated images with unequal defocus distances. This add-on module does not include any lenses or gratings and is cost-effective and easy-to-alignment. The validity of this method is confirmed by the measurement of microlens array and human osteoblastic cells in culture, indicating its potential in the applications of dynamically measuring living cells and other transparent specimens in a quantitative, non-invasive and label-free manner.

Original languageEnglish
Pages (from-to)586-593
Number of pages8
JournalOptics Express
Volume26
Issue number1
DOIs
StatePublished - 8 Jan 2018

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