Preparation of lipase/Zn3(PO4)2 hybrid nanoflower and its catalytic performance as an immobilized enzyme

Baoliang Zhang; Peitao Li; Hepeng Zhang; Hai Wang; Xiangjie Li; Lei Tian; Nisar Ali; Zafar Ali; Qiuyu Zhang

doi:10.1016/j.cej.2016.01.104

Preparation of lipase/Zn₃(PO₄)₂ hybrid nanoflower and its catalytic performance as an immobilized enzyme

Baoliang Zhang, Peitao Li, Hepeng Zhang, Hai Wang, Xiangjie Li, Lei Tian, Nisar Ali, Zafar Ali, Qiuyu Zhang

School of Chemistry and Chemical Engineering

Northwestern Polytechnical University Xian

Research output: Contribution to journal › Article › peer-review

193 Scopus citations

Abstract

A facile and rapid method is reported in this paper to prepare a novel immobilized enzyme named lipase/Zn₃(PO₄)₂ hybrid nanoflower. The growth mechanism of the nanoflower has been studied in detail and can be described as the following four steps: crystallization and coordination, in-situ precipitation, self-assembly, size growth. Addition amount of lipase, reaction temperature and stirring form affect the morphology and lipase content of the hybrid nanoflower. Besides, the catalytic performance of lipase/Zn₃(PO₄)₂ hybrid nanoflower was investigated and the optimal catalytic conditions have been found. The maximum enzyme activity was 855 ± 13 U/g. In comparison with the free lipase, the enzyme activity increment of hybrid nanoflower is 147%. Meanwhile, the unique nanostructure makes lipase/Zn₃(PO₄)₂ hybrid nanoflower an excellent operational stabilizer. The results indicate that the well-designed materials should be useful in industrial enzyme catalysis.

Original language	English
Pages (from-to)	287-297
Number of pages	11
Journal	Chemical Engineering Journal
Volume	291
DOIs	https://doi.org/10.1016/j.cej.2016.01.104
State	Published - 1 May 2016

Keywords

Coordination
Hybrid nanoflower
Immobilized enzyme
Lipase
Precipitation reaction

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title = "Preparation of lipase/Zn3(PO4)2 hybrid nanoflower and its catalytic performance as an immobilized enzyme",

abstract = "A facile and rapid method is reported in this paper to prepare a novel immobilized enzyme named lipase/Zn3(PO4)2 hybrid nanoflower. The growth mechanism of the nanoflower has been studied in detail and can be described as the following four steps: crystallization and coordination, in-situ precipitation, self-assembly, size growth. Addition amount of lipase, reaction temperature and stirring form affect the morphology and lipase content of the hybrid nanoflower. Besides, the catalytic performance of lipase/Zn3(PO4)2 hybrid nanoflower was investigated and the optimal catalytic conditions have been found. The maximum enzyme activity was 855 ± 13 U/g. In comparison with the free lipase, the enzyme activity increment of hybrid nanoflower is 147%. Meanwhile, the unique nanostructure makes lipase/Zn3(PO4)2 hybrid nanoflower an excellent operational stabilizer. The results indicate that the well-designed materials should be useful in industrial enzyme catalysis.",

keywords = "Coordination, Hybrid nanoflower, Immobilized enzyme, Lipase, Precipitation reaction",

author = "Baoliang Zhang and Peitao Li and Hepeng Zhang and Hai Wang and Xiangjie Li and Lei Tian and Nisar Ali and Zafar Ali and Qiuyu Zhang",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = may,

day = "1",

doi = "10.1016/j.cej.2016.01.104",

language = "英语",

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TY - JOUR

T1 - Preparation of lipase/Zn3(PO4)2 hybrid nanoflower and its catalytic performance as an immobilized enzyme

AU - Zhang, Baoliang

AU - Li, Peitao

AU - Zhang, Hepeng

AU - Wang, Hai

AU - Li, Xiangjie

AU - Tian, Lei

AU - Ali, Nisar

AU - Ali, Zafar

AU - Zhang, Qiuyu

PY - 2016/5/1

Y1 - 2016/5/1

N2 - A facile and rapid method is reported in this paper to prepare a novel immobilized enzyme named lipase/Zn3(PO4)2 hybrid nanoflower. The growth mechanism of the nanoflower has been studied in detail and can be described as the following four steps: crystallization and coordination, in-situ precipitation, self-assembly, size growth. Addition amount of lipase, reaction temperature and stirring form affect the morphology and lipase content of the hybrid nanoflower. Besides, the catalytic performance of lipase/Zn3(PO4)2 hybrid nanoflower was investigated and the optimal catalytic conditions have been found. The maximum enzyme activity was 855 ± 13 U/g. In comparison with the free lipase, the enzyme activity increment of hybrid nanoflower is 147%. Meanwhile, the unique nanostructure makes lipase/Zn3(PO4)2 hybrid nanoflower an excellent operational stabilizer. The results indicate that the well-designed materials should be useful in industrial enzyme catalysis.

AB - A facile and rapid method is reported in this paper to prepare a novel immobilized enzyme named lipase/Zn3(PO4)2 hybrid nanoflower. The growth mechanism of the nanoflower has been studied in detail and can be described as the following four steps: crystallization and coordination, in-situ precipitation, self-assembly, size growth. Addition amount of lipase, reaction temperature and stirring form affect the morphology and lipase content of the hybrid nanoflower. Besides, the catalytic performance of lipase/Zn3(PO4)2 hybrid nanoflower was investigated and the optimal catalytic conditions have been found. The maximum enzyme activity was 855 ± 13 U/g. In comparison with the free lipase, the enzyme activity increment of hybrid nanoflower is 147%. Meanwhile, the unique nanostructure makes lipase/Zn3(PO4)2 hybrid nanoflower an excellent operational stabilizer. The results indicate that the well-designed materials should be useful in industrial enzyme catalysis.

KW - Coordination

KW - Hybrid nanoflower

KW - Immobilized enzyme

KW - Lipase

KW - Precipitation reaction

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U2 - 10.1016/j.cej.2016.01.104

DO - 10.1016/j.cej.2016.01.104

M3 - 文章

AN - SCOPUS:84958257007

SN - 1385-8947

VL - 291

SP - 287

EP - 297

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

ER -

Preparation of lipase/Zn₃(PO₄)₂ hybrid nanoflower and its catalytic performance as an immobilized enzyme

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Keywords

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