Photocontrollable Fluorogenic Probe for Visualizing Near-Membrane Hypochlorite in Live Cells

Pingping Peng; Hao Li; Lei Bai; Liulin Wang; Buxiang Chen; Changmin Yu; Chengwu Zhang; Jingyan Ge; Lin Li; Wei Huang

doi:10.1002/slct.201800777

Photocontrollable Fluorogenic Probe for Visualizing Near-Membrane Hypochlorite in Live Cells

Pingping Peng, Hao Li, Lei Bai, Liulin Wang, Buxiang Chen, Changmin Yu, Chengwu Zhang, Jingyan Ge, Lin Li, Wei Huang

Institute of Flexible Electronics

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Hypochlorous acid or hypochlorite (HClO/ClO⁻) near membrane is one of the most critical reactive oxygen species (ROS). Herein, a photocontrollable fluorogenic probe for directly detecting ClO⁻ near membrane has been designed and synthesized. This probe HOCMem was constructed based on fluorescein by incorporation of a photolabile group (nitrobenzyl group), membrane-anchoring unit (cholesterol) and ClO⁻-sensitive moiety (benzoyl group). Upon the UV irradiation, the probe exhibited a significantly increased emission at 520 nm in the presence of ClO⁻. However, no fluorescence was observed with the treatment of only UV irradiation or ClO⁻. In addition, this new probe displayed a good linear relationship with the concentration of ClO⁻ ranged from 1 to 50 μM with a detection limit of 260.77 nM, as well as excellent selectivity for ClO⁻ over other analytes. Furthermore, cell-based experiments showed the low cytotoxicity of this probe and its capacity for directly detecting near-membrane ClO⁻ with light switching on/off.

Original language	English
Pages (from-to)	5981-5986
Number of pages	6
Journal	ChemistrySelect
Volume	3
Issue number	21
DOIs	https://doi.org/10.1002/slct.201800777
State	Published - 7 Jun 2018

Keywords

Cell-membrane
Fluorescence imaging
Fluorogenic probe
Hypochlorite
Photocontrollable

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title = "Photocontrollable Fluorogenic Probe for Visualizing Near-Membrane Hypochlorite in Live Cells",

abstract = "Hypochlorous acid or hypochlorite (HClO/ClO−) near membrane is one of the most critical reactive oxygen species (ROS). Herein, a photocontrollable fluorogenic probe for directly detecting ClO− near membrane has been designed and synthesized. This probe HOCMem was constructed based on fluorescein by incorporation of a photolabile group (nitrobenzyl group), membrane-anchoring unit (cholesterol) and ClO−-sensitive moiety (benzoyl group). Upon the UV irradiation, the probe exhibited a significantly increased emission at 520 nm in the presence of ClO−. However, no fluorescence was observed with the treatment of only UV irradiation or ClO−. In addition, this new probe displayed a good linear relationship with the concentration of ClO− ranged from 1 to 50 μM with a detection limit of 260.77 nM, as well as excellent selectivity for ClO− over other analytes. Furthermore, cell-based experiments showed the low cytotoxicity of this probe and its capacity for directly detecting near-membrane ClO− with light switching on/off.",

keywords = "Cell-membrane, Fluorescence imaging, Fluorogenic probe, Hypochlorite, Photocontrollable",

author = "Pingping Peng and Hao Li and Lei Bai and Liulin Wang and Buxiang Chen and Changmin Yu and Chengwu Zhang and Jingyan Ge and Lin Li and Wei Huang",

note = "Publisher Copyright: {\textcopyright} 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2018",

month = jun,

day = "7",

doi = "10.1002/slct.201800777",

language = "英语",

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T1 - Photocontrollable Fluorogenic Probe for Visualizing Near-Membrane Hypochlorite in Live Cells

AU - Peng, Pingping

AU - Li, Hao

AU - Bai, Lei

AU - Wang, Liulin

AU - Chen, Buxiang

AU - Yu, Changmin

AU - Zhang, Chengwu

AU - Ge, Jingyan

AU - Li, Lin

AU - Huang, Wei

PY - 2018/6/7

Y1 - 2018/6/7

N2 - Hypochlorous acid or hypochlorite (HClO/ClO−) near membrane is one of the most critical reactive oxygen species (ROS). Herein, a photocontrollable fluorogenic probe for directly detecting ClO− near membrane has been designed and synthesized. This probe HOCMem was constructed based on fluorescein by incorporation of a photolabile group (nitrobenzyl group), membrane-anchoring unit (cholesterol) and ClO−-sensitive moiety (benzoyl group). Upon the UV irradiation, the probe exhibited a significantly increased emission at 520 nm in the presence of ClO−. However, no fluorescence was observed with the treatment of only UV irradiation or ClO−. In addition, this new probe displayed a good linear relationship with the concentration of ClO− ranged from 1 to 50 μM with a detection limit of 260.77 nM, as well as excellent selectivity for ClO− over other analytes. Furthermore, cell-based experiments showed the low cytotoxicity of this probe and its capacity for directly detecting near-membrane ClO− with light switching on/off.

AB - Hypochlorous acid or hypochlorite (HClO/ClO−) near membrane is one of the most critical reactive oxygen species (ROS). Herein, a photocontrollable fluorogenic probe for directly detecting ClO− near membrane has been designed and synthesized. This probe HOCMem was constructed based on fluorescein by incorporation of a photolabile group (nitrobenzyl group), membrane-anchoring unit (cholesterol) and ClO−-sensitive moiety (benzoyl group). Upon the UV irradiation, the probe exhibited a significantly increased emission at 520 nm in the presence of ClO−. However, no fluorescence was observed with the treatment of only UV irradiation or ClO−. In addition, this new probe displayed a good linear relationship with the concentration of ClO− ranged from 1 to 50 μM with a detection limit of 260.77 nM, as well as excellent selectivity for ClO− over other analytes. Furthermore, cell-based experiments showed the low cytotoxicity of this probe and its capacity for directly detecting near-membrane ClO− with light switching on/off.

KW - Cell-membrane

KW - Fluorescence imaging

KW - Fluorogenic probe

KW - Hypochlorite

KW - Photocontrollable

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U2 - 10.1002/slct.201800777

DO - 10.1002/slct.201800777

M3 - 文章

AN - SCOPUS:85048321201

SN - 2365-6549

VL - 3

SP - 5981

EP - 5986

JO - ChemistrySelect

JF - ChemistrySelect

IS - 21

ER -

Photocontrollable Fluorogenic Probe for Visualizing Near-Membrane Hypochlorite in Live Cells

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