Methods of studying mammalian cell migration and invasion in vitro

Peihong Su; Zhiping Miao; Lifang Hu; Runzhi Li; Chong Yin; Dijie Li; Zhihao Chen; Fan Zhao; Airong Qian

doi:10.1109/IBCAST.2017.7868048

Methods of studying mammalian cell migration and invasion in vitro

Peihong Su, Zhiping Miao, Lifang Hu, Runzhi Li, Chong Yin, Dijie Li, Zhihao Chen, Fan Zhao, Airong Qian

School of life sciences

Northwestern Polytechnical University Xian

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

Cell migration is a critical event for many physiological processes, such as embryonic development, renewal of skin and intestine, tissue repair, immune surveillance and so on. The abnormality of cell migration affects normal physiological processes and causes diseases, such as wound-healing delay of skin. Cell culture in vitro is essential for studying the migration process and the underlying mechanism. For cell migration detection in vitro, there are a number of methods and each has its own advantages and limitations. Transwell and wound healing assay are the most common methods used to study migration of different cells under different conditions. Besides, other assays are developed in the process of studying cell migration. In recently years, 3D cell migration attracts more and more attention for its accurate mimic of in vivo environment and it may be a trend of detecting cell migration and invasion in vitro in future. This paper summarizes current methods of studying cell migration in vitro and provides reference to researchers.

Original language	English
Title of host publication	Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
Editors	Muhammad Zafar-uz-Zaman
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	148-159
Number of pages	12
ISBN (Electronic)	9781467390736
DOIs	https://doi.org/10.1109/IBCAST.2017.7868048
State	Published - 1 Mar 2017
Event	14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017 - Islamabad, Pakistan Duration: 10 Jan 2017 → 14 Jan 2017

Publication series

Name	Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017

Conference

Conference	14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
Country/Territory	Pakistan
City	Islamabad
Period	10/01/17 → 14/01/17

Keywords

cell migration
chemotaxis
in vitro
invasion
wound healing

Access to Document

10.1109/IBCAST.2017.7868048

Cite this

Su, P., Miao, Z., Hu, L., Li, R., Yin, C., Li, D., Chen, Z., Zhao, F., & Qian, A. (2017). Methods of studying mammalian cell migration and invasion in vitro. In M. Zafar-uz-Zaman (Ed.), Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017 (pp. 148-159). Article 7868048 (Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IBCAST.2017.7868048

Su, Peihong ; Miao, Zhiping ; Hu, Lifang et al. / Methods of studying mammalian cell migration and invasion in vitro. Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017. editor / Muhammad Zafar-uz-Zaman. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 148-159 (Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017).

@inproceedings{dbd5eb0399ef4599bc765384fa837d30,

title = "Methods of studying mammalian cell migration and invasion in vitro",

abstract = "Cell migration is a critical event for many physiological processes, such as embryonic development, renewal of skin and intestine, tissue repair, immune surveillance and so on. The abnormality of cell migration affects normal physiological processes and causes diseases, such as wound-healing delay of skin. Cell culture in vitro is essential for studying the migration process and the underlying mechanism. For cell migration detection in vitro, there are a number of methods and each has its own advantages and limitations. Transwell and wound healing assay are the most common methods used to study migration of different cells under different conditions. Besides, other assays are developed in the process of studying cell migration. In recently years, 3D cell migration attracts more and more attention for its accurate mimic of in vivo environment and it may be a trend of detecting cell migration and invasion in vitro in future. This paper summarizes current methods of studying cell migration in vitro and provides reference to researchers.",

keywords = "cell migration, chemotaxis, in vitro, invasion, wound healing",

author = "Peihong Su and Zhiping Miao and Lifang Hu and Runzhi Li and Chong Yin and Dijie Li and Zhihao Chen and Fan Zhao and Airong Qian",

note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017 ; Conference date: 10-01-2017 Through 14-01-2017",

year = "2017",

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doi = "10.1109/IBCAST.2017.7868048",

language = "英语",

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Su, P, Miao, Z, Hu, L, Li, R, Yin, C, Li, D, Chen, Z, Zhao, F & Qian, A 2017, Methods of studying mammalian cell migration and invasion in vitro. in M Zafar-uz-Zaman (ed.), Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017., 7868048, Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017, Institute of Electrical and Electronics Engineers Inc., pp. 148-159, 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017, Islamabad, Pakistan, 10/01/17. https://doi.org/10.1109/IBCAST.2017.7868048

Methods of studying mammalian cell migration and invasion in vitro. / Su, Peihong; Miao, Zhiping; Hu, Lifang et al.
Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017. ed. / Muhammad Zafar-uz-Zaman. Institute of Electrical and Electronics Engineers Inc., 2017. p. 148-159 7868048 (Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Methods of studying mammalian cell migration and invasion in vitro

AU - Su, Peihong

AU - Miao, Zhiping

AU - Hu, Lifang

AU - Li, Runzhi

AU - Yin, Chong

AU - Li, Dijie

AU - Chen, Zhihao

AU - Zhao, Fan

AU - Qian, Airong

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Cell migration is a critical event for many physiological processes, such as embryonic development, renewal of skin and intestine, tissue repair, immune surveillance and so on. The abnormality of cell migration affects normal physiological processes and causes diseases, such as wound-healing delay of skin. Cell culture in vitro is essential for studying the migration process and the underlying mechanism. For cell migration detection in vitro, there are a number of methods and each has its own advantages and limitations. Transwell and wound healing assay are the most common methods used to study migration of different cells under different conditions. Besides, other assays are developed in the process of studying cell migration. In recently years, 3D cell migration attracts more and more attention for its accurate mimic of in vivo environment and it may be a trend of detecting cell migration and invasion in vitro in future. This paper summarizes current methods of studying cell migration in vitro and provides reference to researchers.

AB - Cell migration is a critical event for many physiological processes, such as embryonic development, renewal of skin and intestine, tissue repair, immune surveillance and so on. The abnormality of cell migration affects normal physiological processes and causes diseases, such as wound-healing delay of skin. Cell culture in vitro is essential for studying the migration process and the underlying mechanism. For cell migration detection in vitro, there are a number of methods and each has its own advantages and limitations. Transwell and wound healing assay are the most common methods used to study migration of different cells under different conditions. Besides, other assays are developed in the process of studying cell migration. In recently years, 3D cell migration attracts more and more attention for its accurate mimic of in vivo environment and it may be a trend of detecting cell migration and invasion in vitro in future. This paper summarizes current methods of studying cell migration in vitro and provides reference to researchers.

KW - cell migration

KW - chemotaxis

KW - in vitro

KW - invasion

KW - wound healing

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BT - Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017

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PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017

Y2 - 10 January 2017 through 14 January 2017

ER -

Su P, Miao Z, Hu L, Li R, Yin C, Li D et al. Methods of studying mammalian cell migration and invasion in vitro. In Zafar-uz-Zaman M, editor, Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 148-159. 7868048. (Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017). doi: 10.1109/IBCAST.2017.7868048

Methods of studying mammalian cell migration and invasion in vitro

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