Abstract
The dynamic analysis of biological living samples is one of the particular interests in life sciences. An improved digital holographic microscope for long-term quantitative phase-contrast imaging of living cells is presented in this paper. The optical configuration is optimized in the form of a free-space-fiber hybrid system which promotes the flexibility of imaging in complex or semi-enclosed experimental environment. Aberrations compensation is implemented taking into account the additional phase aberration induced by liquid culture medium in long-term observation. The proposed approach is applied to investigate living samples of MC3T3-E1 and MLO-Y4 cells. The experimental results demonstrate its availability in the analysis of cellular changes.
| Original language | English |
|---|---|
| Pages (from-to) | 740-745 |
| Number of pages | 6 |
| Journal | Laser Physics |
| Volume | 21 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2011 |