Comparison of the quality of protein crystals grown by CLPC seeds method

Jin Li; Er Kai Yan; Yue Liu; Zi Qing Wu; Ya Li Liu; Hai Hou; Chen Yan Zhang; Qin Qin Lu; Xu Dong Deng; Da Chuan Yin

doi:10.3390/cryst9100501

Comparison of the quality of protein crystals grown by CLPC seeds method

Jin Li, Er Kai Yan, Yue Liu, Zi Qing Wu, Ya Li Liu, Hai Hou, Chen Yan Zhang, Qin Qin Lu, Xu Dong Deng, Da Chuan Yin

School of life sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.

Original language	English
Article number	501
Journal	Crystals
Volume	9
Issue number	10
DOIs	https://doi.org/10.3390/cryst9100501
State	Published - Oct 2019

Keywords

CLPC seeds
Crystal quality
Mosaicity
Resolution

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10.3390/cryst9100501

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@article{d5adde50ba594885ad19f3a0cf183e27,

title = "Comparison of the quality of protein crystals grown by CLPC seeds method",

abstract = "We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.",

keywords = "CLPC seeds, Crystal quality, Mosaicity, Resolution",

author = "Jin Li and Yan, {Er Kai} and Yue Liu and Wu, {Zi Qing} and Liu, {Ya Li} and Hai Hou and Zhang, {Chen Yan} and Lu, {Qin Qin} and Deng, {Xu Dong} and Yin, {Da Chuan}",

note = "Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

month = oct,

doi = "10.3390/cryst9100501",

language = "英语",

volume = "9",

journal = "Crystals",

issn = "2073-4352",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "10",

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TY - JOUR

T1 - Comparison of the quality of protein crystals grown by CLPC seeds method

AU - Li, Jin

AU - Yan, Er Kai

AU - Liu, Yue

AU - Wu, Zi Qing

AU - Liu, Ya Li

AU - Hou, Hai

AU - Zhang, Chen Yan

AU - Lu, Qin Qin

AU - Deng, Xu Dong

AU - Yin, Da Chuan

PY - 2019/10

Y1 - 2019/10

N2 - We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.

AB - We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.

KW - CLPC seeds

KW - Crystal quality

KW - Mosaicity

KW - Resolution

UR - http://www.scopus.com/inward/record.url?scp=85073423558&partnerID=8YFLogxK

U2 - 10.3390/cryst9100501

DO - 10.3390/cryst9100501

M3 - 文章

AN - SCOPUS:85073423558

SN - 2073-4352

VL - 9

JO - Crystals

JF - Crystals

IS - 10

M1 - 501

ER -

Comparison of the quality of protein crystals grown by CLPC seeds method

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Keywords

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