Classification of cell morphology with quantitative phase microscopy and machine learning

Ying Li, Jianglei Di, Kaiqiang Wang, Sufang Wang, Jianlin Zhao

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We describe and compare two machine learning approaches for cell classification based on label-free quantitative phase imaging with transport of intensity equation methods. In one approach, we design a multilevel integrated machine learning classifier including various individual models such as artificial neural network, extreme learning machine and generalized logistic regression. In another approach, we apply a pretrained convolutional neural network using transfer learning for the classification. As a validation, we show the performances of both approaches on classification between macrophages cultured in normal gravity and microgravity with quantitative phase imaging. The multilevel integrated classifier achieves average accuracy 93.1%, which is comparable to the average accuracy 93.5% obtained by convolutional neural network. The presented quantitative phase imaging system with two classification approaches could be helpful to biomedical scientists for easy and accurate cell analysis.

Original languageEnglish
Pages (from-to)23916-23927
Number of pages12
JournalOptics Express
Volume28
Issue number16
DOIs
StatePublished - 3 Aug 2020

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