All-printed point-of-care immunosensing biochip for one drop blood diagnostics

Jimei Chi; Yuanbin Wu; Feifei Qin; Meng Su; Nan Cheng; Jiabing Zhang; Chunbao Li; Zewei Lian; Xu Yang; Lijun Cheng; Hongfei Xie; Huadong Wang; Zeying Zhang; Jan Carmeliet; Yanlin Song

doi:10.1039/d2lc00385f

All-printed point-of-care immunosensing biochip for one drop blood diagnostics

Jimei Chi, Yuanbin Wu, Feifei Qin, Meng Su, Nan Cheng, Jiabing Zhang, Chunbao Li, Zewei Lian, Xu Yang, Lijun Cheng, Hongfei Xie, Huadong Wang, Zeying Zhang, Jan Carmeliet, Yanlin Song

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Designing and preparing a fast and easy-to-use immunosensing biochip are of great significance for clinical diagnosis and biomedical research. In particular, sensitive, specific, and early detection of biomarkers in trace samples promotes the application of point-of-care testing (POCT). Here, we demonstrate an all-printed immunosensing biochip with the characteristics of hydrodynamic enrichment and photonic crystal-enhanced fluorescence. Direct quantitative detection of cardiac biomarkers via one drop of blood is achieved in 10 min. After simulating the hydrodynamic behavior of one droplet serum on the printed assay, creatine kinase-MB (CK-MB) has been recognized and located on the photonic crystal arrays. Benefiting from the fluorescence enhancement effect, quantitative detection of CK-MB has been demonstrated from 0.01 ng ml⁻¹ to 100 ng ml⁻¹, which is superior to the conventional enzyme-linked immunosorbent assay (ELISA). This strategy provides a general and easy-to-use approach for fast quantitative detection of biomarkers, which would be improved further for portable clinical diagnostics and home medical monitoring.

Original language	English
Pages (from-to)	3008-3014
Number of pages	7
Journal	Lab on a Chip
Volume	22
Issue number	16
DOIs	https://doi.org/10.1039/d2lc00385f
State	Published - 21 Jun 2022
Externally published	Yes

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title = "All-printed point-of-care immunosensing biochip for one drop blood diagnostics",

abstract = "Designing and preparing a fast and easy-to-use immunosensing biochip are of great significance for clinical diagnosis and biomedical research. In particular, sensitive, specific, and early detection of biomarkers in trace samples promotes the application of point-of-care testing (POCT). Here, we demonstrate an all-printed immunosensing biochip with the characteristics of hydrodynamic enrichment and photonic crystal-enhanced fluorescence. Direct quantitative detection of cardiac biomarkers via one drop of blood is achieved in 10 min. After simulating the hydrodynamic behavior of one droplet serum on the printed assay, creatine kinase-MB (CK-MB) has been recognized and located on the photonic crystal arrays. Benefiting from the fluorescence enhancement effect, quantitative detection of CK-MB has been demonstrated from 0.01 ng ml−1 to 100 ng ml−1, which is superior to the conventional enzyme-linked immunosorbent assay (ELISA). This strategy provides a general and easy-to-use approach for fast quantitative detection of biomarkers, which would be improved further for portable clinical diagnostics and home medical monitoring.",

author = "Jimei Chi and Yuanbin Wu and Feifei Qin and Meng Su and Nan Cheng and Jiabing Zhang and Chunbao Li and Zewei Lian and Xu Yang and Lijun Cheng and Hongfei Xie and Huadong Wang and Zeying Zhang and Jan Carmeliet and Yanlin Song",

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doi = "10.1039/d2lc00385f",

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T1 - All-printed point-of-care immunosensing biochip for one drop blood diagnostics

AU - Chi, Jimei

AU - Wu, Yuanbin

AU - Qin, Feifei

AU - Su, Meng

AU - Cheng, Nan

AU - Zhang, Jiabing

AU - Li, Chunbao

AU - Lian, Zewei

AU - Yang, Xu

AU - Cheng, Lijun

AU - Xie, Hongfei

AU - Wang, Huadong

AU - Zhang, Zeying

AU - Carmeliet, Jan

AU - Song, Yanlin

PY - 2022/6/21

Y1 - 2022/6/21

N2 - Designing and preparing a fast and easy-to-use immunosensing biochip are of great significance for clinical diagnosis and biomedical research. In particular, sensitive, specific, and early detection of biomarkers in trace samples promotes the application of point-of-care testing (POCT). Here, we demonstrate an all-printed immunosensing biochip with the characteristics of hydrodynamic enrichment and photonic crystal-enhanced fluorescence. Direct quantitative detection of cardiac biomarkers via one drop of blood is achieved in 10 min. After simulating the hydrodynamic behavior of one droplet serum on the printed assay, creatine kinase-MB (CK-MB) has been recognized and located on the photonic crystal arrays. Benefiting from the fluorescence enhancement effect, quantitative detection of CK-MB has been demonstrated from 0.01 ng ml−1 to 100 ng ml−1, which is superior to the conventional enzyme-linked immunosorbent assay (ELISA). This strategy provides a general and easy-to-use approach for fast quantitative detection of biomarkers, which would be improved further for portable clinical diagnostics and home medical monitoring.

AB - Designing and preparing a fast and easy-to-use immunosensing biochip are of great significance for clinical diagnosis and biomedical research. In particular, sensitive, specific, and early detection of biomarkers in trace samples promotes the application of point-of-care testing (POCT). Here, we demonstrate an all-printed immunosensing biochip with the characteristics of hydrodynamic enrichment and photonic crystal-enhanced fluorescence. Direct quantitative detection of cardiac biomarkers via one drop of blood is achieved in 10 min. After simulating the hydrodynamic behavior of one droplet serum on the printed assay, creatine kinase-MB (CK-MB) has been recognized and located on the photonic crystal arrays. Benefiting from the fluorescence enhancement effect, quantitative detection of CK-MB has been demonstrated from 0.01 ng ml−1 to 100 ng ml−1, which is superior to the conventional enzyme-linked immunosorbent assay (ELISA). This strategy provides a general and easy-to-use approach for fast quantitative detection of biomarkers, which would be improved further for portable clinical diagnostics and home medical monitoring.

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U2 - 10.1039/d2lc00385f

DO - 10.1039/d2lc00385f

M3 - 文章

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AN - SCOPUS:85135768793

SN - 1473-0197

VL - 22

SP - 3008

EP - 3014

JO - Lab on a Chip

JF - Lab on a Chip

IS - 16

ER -

All-printed point-of-care immunosensing biochip for one drop blood diagnostics

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