TY - JOUR
T1 - A novel fluorogenic probe for visualizing the hydrogen peroxide in Parkinson's disease models
AU - Zhang, Gaobin
AU - Li, Zheng
AU - Chen, Fangjie
AU - Zhang, Duoteng
AU - Ji, Wenhui
AU - Yang, Zhengpeng
AU - Wu, Qiong
AU - Zhang, Chengwu
AU - Li, Lin
AU - Huang, Wei
N1 - Publisher Copyright:
© 2020 The Author(s).
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Parkinson's disease (PD) is closely related to the oxidative stress induced by excess hydrogen peroxide (H2O2) in organisms. Developing an efficient method for noninvasive and real-time H2O2 detection is beneficial to investigate the role played by H2O2 in PD. In this work, a novel fluorogenic probe (CBH) for living organisms H2O2 detection has been designed, synthesized and characterized. The emission of CBH in PBS solution is very weak. However, when H2O2 was added, the fluorescence of CBH solution was sharply increased for 12-fold, accompanied by the emission peak blue-shifted from 600 to 530 nm. Moreover, the response of CBH to H2O2 is highly sensitive and selective and is not affected by various ROS/RNS, anions, cations, and amino acids. Based on the good performance of CBH for H2O2 detection, it has been successfully applied to visualizing the H2O2 concentration in living cells, Zebrafish and C. elegans PD models.
AB - Parkinson's disease (PD) is closely related to the oxidative stress induced by excess hydrogen peroxide (H2O2) in organisms. Developing an efficient method for noninvasive and real-time H2O2 detection is beneficial to investigate the role played by H2O2 in PD. In this work, a novel fluorogenic probe (CBH) for living organisms H2O2 detection has been designed, synthesized and characterized. The emission of CBH in PBS solution is very weak. However, when H2O2 was added, the fluorescence of CBH solution was sharply increased for 12-fold, accompanied by the emission peak blue-shifted from 600 to 530 nm. Moreover, the response of CBH to H2O2 is highly sensitive and selective and is not affected by various ROS/RNS, anions, cations, and amino acids. Based on the good performance of CBH for H2O2 detection, it has been successfully applied to visualizing the H2O2 concentration in living cells, Zebrafish and C. elegans PD models.
KW - Fluorescence
KW - Parkinson's disease
KW - chemosensor
KW - hydroperoxide
UR - http://www.scopus.com/inward/record.url?scp=85084437276&partnerID=8YFLogxK
U2 - 10.1142/S1793545820500133
DO - 10.1142/S1793545820500133
M3 - 文章
AN - SCOPUS:85084437276
SN - 1793-5458
VL - 13
JO - Journal of Innovative Optical Health Sciences
JF - Journal of Innovative Optical Health Sciences
IS - 3
M1 - 2050013
ER -