A Microflow Cytometer Based on a Disposable Microfluidic Chip With Side Scatter and Fluorescence Detection Capability

Wenpeng Xun; Jianguo Feng; Honglong Chang

doi:10.1109/TNB.2015.2455073

A Microflow Cytometer Based on a Disposable Microfluidic Chip With Side Scatter and Fluorescence Detection Capability

Wenpeng Xun, Jianguo Feng, Honglong Chang

School of Mechanical Engineering

Northwestern Polytechnical University Xian

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

In this paper, we present a microflow cytometer based on a polydimethylsiloxane (PDMS) microfluidic chip. The PDMS chip has a smooth sidewall because of the proposed double molding process. Thus, the side scatter (SSC) detection can be used for the cytometer. The coincidence error using the sheathless focus mechanism is less than 0.069%. Using Flow-Check alignment beads for the counting experiment, the coefficient of variation (CV) of the SSC and the fluorescein isothiocyanate (FITC) channel can reach 8.37% and 2.46%, respectively. The gating of the lymphocytes according to CD45/SSC was also demonstrated successfully. It proves that the chip is a promising solution for point of care (POC) microflow cytometers.

Original language	English
Article number	7277090
Pages (from-to)	850-856
Number of pages	7
Journal	IEEE Transactions on Nanobioscience
Volume	14
Issue number	8
DOIs	https://doi.org/10.1109/TNB.2015.2455073
State	Published - Dec 2015

Keywords

Flow cytometer
fluorescence
microfluidics
polydimethylsiloxane
side scatter

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10.1109/TNB.2015.2455073

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title = "A Microflow Cytometer Based on a Disposable Microfluidic Chip With Side Scatter and Fluorescence Detection Capability",

abstract = "In this paper, we present a microflow cytometer based on a polydimethylsiloxane (PDMS) microfluidic chip. The PDMS chip has a smooth sidewall because of the proposed double molding process. Thus, the side scatter (SSC) detection can be used for the cytometer. The coincidence error using the sheathless focus mechanism is less than 0.069%. Using Flow-Check alignment beads for the counting experiment, the coefficient of variation (CV) of the SSC and the fluorescein isothiocyanate (FITC) channel can reach 8.37% and 2.46%, respectively. The gating of the lymphocytes according to CD45/SSC was also demonstrated successfully. It proves that the chip is a promising solution for point of care (POC) microflow cytometers.",

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author = "Wenpeng Xun and Jianguo Feng and Honglong Chang",

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AU - Feng, Jianguo

AU - Chang, Honglong

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AB - In this paper, we present a microflow cytometer based on a polydimethylsiloxane (PDMS) microfluidic chip. The PDMS chip has a smooth sidewall because of the proposed double molding process. Thus, the side scatter (SSC) detection can be used for the cytometer. The coincidence error using the sheathless focus mechanism is less than 0.069%. Using Flow-Check alignment beads for the counting experiment, the coefficient of variation (CV) of the SSC and the fluorescein isothiocyanate (FITC) channel can reach 8.37% and 2.46%, respectively. The gating of the lymphocytes according to CD45/SSC was also demonstrated successfully. It proves that the chip is a promising solution for point of care (POC) microflow cytometers.

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A Microflow Cytometer Based on a Disposable Microfluidic Chip With Side Scatter and Fluorescence Detection Capability

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