A Microflow Cytometer Based on a Disposable Microfluidic Chip With Side Scatter and Fluorescence Detection Capability

Wenpeng Xun, Jianguo Feng, Honglong Chang

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

In this paper, we present a microflow cytometer based on a polydimethylsiloxane (PDMS) microfluidic chip. The PDMS chip has a smooth sidewall because of the proposed double molding process. Thus, the side scatter (SSC) detection can be used for the cytometer. The coincidence error using the sheathless focus mechanism is less than 0.069%. Using Flow-Check alignment beads for the counting experiment, the coefficient of variation (CV) of the SSC and the fluorescein isothiocyanate (FITC) channel can reach 8.37% and 2.46%, respectively. The gating of the lymphocytes according to CD45/SSC was also demonstrated successfully. It proves that the chip is a promising solution for point of care (POC) microflow cytometers.

Original languageEnglish
Article number7277090
Pages (from-to)850-856
Number of pages7
JournalIEEE Transactions on Nanobioscience
Volume14
Issue number8
DOIs
StatePublished - Dec 2015

Keywords

  • Flow cytometer
  • fluorescence
  • microfluidics
  • polydimethylsiloxane
  • side scatter

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