A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods

Hai Hou; Bo Wang; Shan Yang Hu; Jing Zhang Wang; Peng Fei Zhu; Yue Liu; Meng Ying Wang; Da Chuan Yin

doi:10.1039/c5ce00551e

A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods

Hai Hou, Bo Wang, Shan Yang Hu, Jing Zhang Wang, Peng Fei Zhu, Yue Liu, Meng Ying Wang, Da Chuan Yin

School of life sciences

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.

Original language	English
Pages (from-to)	5365-5371
Number of pages	7
Journal	CrystEngComm
Volume	17
Issue number	29
DOIs	https://doi.org/10.1039/c5ce00551e
State	Published - 7 Aug 2015

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title = "A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods",

abstract = "We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.",

author = "Hai Hou and Bo Wang and Hu, {Shan Yang} and Wang, {Jing Zhang} and Zhu, {Peng Fei} and Yue Liu and Wang, {Meng Ying} and Yin, {Da Chuan}",

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AU - Hou, Hai

AU - Wang, Bo

AU - Hu, Shan Yang

AU - Wang, Jing Zhang

AU - Zhu, Peng Fei

AU - Liu, Yue

AU - Wang, Meng Ying

AU - Yin, Da Chuan

PY - 2015/8/7

Y1 - 2015/8/7

N2 - We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.

AB - We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.

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U2 - 10.1039/c5ce00551e

DO - 10.1039/c5ce00551e

M3 - 文章

AN - SCOPUS:84937053863

SN - 1466-8033

VL - 17

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EP - 5371

JO - CrystEngComm

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ER -

A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods

Abstract

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