杜仲协同拟茎点霉 XP-8 合成木脂素类的条件优化及其功能活性

Optimization of process conditions for biosynthesis of pinoresinol (Pin), pinoresinol-4-O-β-D-glucopyranoside (PMG) and pinoresinol diglucoside (PDG) by Phomopsis sp. XP-8 assisted Eucommia ulmoides Oliv. was performed. The effects of the cultured method, the addition of Eucommia ulmoides Oliv. and the time of resting on the production of Pin, PMG and PDG by Phomopsis sp. XP-8 assisted Eucommia ulmoides Oliv. were studied. The antioxidative, anticancer properties of the related products were also investigated. The results showed that the optimal yield of Pin, PMG and PDG was obtained at the condition with the PDB medium contained 3% Eucommia ulmoides Oliv. with resting treatment for 5 d. Under the optimal condition, the free radical scavenging ability for DPPH and ABTS⁺ of metabolite liquid was significantly better than that of the blank culture medium. This indicated that the antioxidative substances were generated during the fermentation process. The hepatocellular carcinoma HepG-2 proliferation was suppressed by the extract solution. The pure product verifications of activity of the standard samples (Pin, PMG and PDG) were performed. It indicated that Pin was identified as the main active ingredient that suppressed HepG-2. The suppressed effect of the metabolite liquid on HepG-2 was a synergistic effect.