Preparation and properties of high-density HBsAg-conjugated magnetic probes

The magnetic beads loaded with high-density Hepatitis B Surface Antigen(HBSAg) detection probes were prepared, and the properties of the beads for HBsAg detection were also studied. The immunomagnetic beads coupled with HBsAb were prepared by chemical bond. Then, immunomagnetic beads were used for detecting HBSAg and were obtained a favorable effect. In order to improve the accuracy and sensitivity of detection, the preparation process of immunomagnetic beads was optimized, including amination and coupling process. Through the optimization, the amino content of magnetic beads was 2.71 mmol/g, unit magne-tic beads bonded 108.36 μg/mg antibodies and the coupling efficiency was 77.40%. The activity of HBsAg on magnetic beads was detected by the method similar to Enzyme-Linked Immunosorbent Assay(ELISA). Compared with common ELISA technology, magnetic beads loaded with high-density HBSAg have higher sensitivity.