TY - JOUR
T1 - Polycystin 2 is involved in the nitric oxide production in responding to oscillating fluid shear in MLO-Y4 cells
AU - Xu, Huiyun
AU - Guan, Ying
AU - Wu, Jiawei
AU - Zhang, Jian
AU - Duan, Jin
AU - An, Long
AU - Shang, Peng
PY - 2014/1/22
Y1 - 2014/1/22
N2 - As a mechano-calcium channel, polycystin2 (PC2) play an important role in the response of renal epithelial cells to fluid flow shear stress. In bone tissue, osteocytes are well known as the main mechanosensory cells, and sensitive to fluid flow stimulus in vitro. In the study, we investigated the effects of oscillating fluid flow (OFF, 2. h, 1. Hz, 1.0. Pa) on the release of Nitric Oxide (NO) and ProstaglandinE2 (PGE2), and the role of PC2 on the release. Our findings demonstrate that PC2 expression increases after 2. h of OFF, and silencing PC2 by RNAi inhibits downstream NO production and iNOS expression, but does not affect the response of PGE2 to OFF.
AB - As a mechano-calcium channel, polycystin2 (PC2) play an important role in the response of renal epithelial cells to fluid flow shear stress. In bone tissue, osteocytes are well known as the main mechanosensory cells, and sensitive to fluid flow stimulus in vitro. In the study, we investigated the effects of oscillating fluid flow (OFF, 2. h, 1. Hz, 1.0. Pa) on the release of Nitric Oxide (NO) and ProstaglandinE2 (PGE2), and the role of PC2 on the release. Our findings demonstrate that PC2 expression increases after 2. h of OFF, and silencing PC2 by RNAi inhibits downstream NO production and iNOS expression, but does not affect the response of PGE2 to OFF.
KW - Nitric oxide
KW - Oscillating fluid flow
KW - Osteocyte
KW - Polycystin2
KW - ProstaglandinE2
UR - http://www.scopus.com/inward/record.url?scp=84891830600&partnerID=8YFLogxK
U2 - 10.1016/j.jbiomech.2013.11.018
DO - 10.1016/j.jbiomech.2013.11.018
M3 - 文章
C2 - 24268313
AN - SCOPUS:84891830600
SN - 0021-9290
VL - 47
SP - 387
EP - 391
JO - Journal of Biomechanics
JF - Journal of Biomechanics
IS - 2
ER -