TY - JOUR
T1 - Phase aberration compensation of digital holographic microscopy based on least squares surface fitting
AU - Di, Jianglei
AU - Zhao, Jianlin
AU - Sun, Weiwei
AU - Jiang, Hongzhen
AU - Yan, Xiaobo
PY - 2009/10/1
Y1 - 2009/10/1
N2 - Digital holographic microscopy allows the numerical reconstruction of the complex wavefront of samples, especially biological samples such as living cells. In digital holographic microscopy, a microscope objective is introduced to improve the transverse resolution of the sample; however a phase aberration in the object wavefront is also brought along, which will affect the phase distribution of the reconstructed image. We propose here a numerical method to compensate for the phase aberration of thin transparent objects with a single hologram. The least squares surface fitting with points number less than the matrix of the original hologram is performed on the unwrapped phase distribution to remove the unwanted wavefront curvature. The proposed method is demonstrated with the samples of the cicada wings and epidermal cells of garlic, and the experimental results are consistent with that of the double exposure method.
AB - Digital holographic microscopy allows the numerical reconstruction of the complex wavefront of samples, especially biological samples such as living cells. In digital holographic microscopy, a microscope objective is introduced to improve the transverse resolution of the sample; however a phase aberration in the object wavefront is also brought along, which will affect the phase distribution of the reconstructed image. We propose here a numerical method to compensate for the phase aberration of thin transparent objects with a single hologram. The least squares surface fitting with points number less than the matrix of the original hologram is performed on the unwrapped phase distribution to remove the unwanted wavefront curvature. The proposed method is demonstrated with the samples of the cicada wings and epidermal cells of garlic, and the experimental results are consistent with that of the double exposure method.
KW - Aberration compensation
KW - Digital holographic microscopy
KW - Least squares method
KW - Phase aberration
KW - Surface fitting
UR - http://www.scopus.com/inward/record.url?scp=68549122667&partnerID=8YFLogxK
U2 - 10.1016/j.optcom.2009.06.049
DO - 10.1016/j.optcom.2009.06.049
M3 - 文章
AN - SCOPUS:68549122667
SN - 0030-4018
VL - 282
SP - 3873
EP - 3877
JO - Optics Communications
JF - Optics Communications
IS - 19
ER -