TY - JOUR
T1 - New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED)
AU - Zhang, Zheng
AU - Yang, Xiangmin
AU - Yang, Hushan
AU - Yu, Xiaoling
AU - Li, Yu
AU - Xing, Jinliang
AU - Chen, Zhinan
PY - 2011/1
Y1 - 2011/1
N2 - HAb18G/CD147, a cancer-associated biomarker, can promote tumor growth, invasion and metastasis. Here, we established a new strategy to express the extracellular domain of HAb18G/CD147 (HAb18GED) by using the FRT/Flp-In recombinase-based site-directed integration system. Two clones expressing HAb18GED were established, and 600. mg HAb18GED was obtained with more than 95% purity. Our results showed that purified HAb18GED exhibited strong activities to stimulate the secretion of pro-MMP2 and MMP-2 and promote the invasion of HCC cells. Overall, our study effectively overcame the difficulty of large-scale HAb18GED preparation and laid a solid foundation for the further studies on structure and functions of HAb18G/CD147.
AB - HAb18G/CD147, a cancer-associated biomarker, can promote tumor growth, invasion and metastasis. Here, we established a new strategy to express the extracellular domain of HAb18G/CD147 (HAb18GED) by using the FRT/Flp-In recombinase-based site-directed integration system. Two clones expressing HAb18GED were established, and 600. mg HAb18GED was obtained with more than 95% purity. Our results showed that purified HAb18GED exhibited strong activities to stimulate the secretion of pro-MMP2 and MMP-2 and promote the invasion of HCC cells. Overall, our study effectively overcame the difficulty of large-scale HAb18GED preparation and laid a solid foundation for the further studies on structure and functions of HAb18G/CD147.
KW - HAb18G/CD147
KW - Protein expression
KW - Site-directed integration system
UR - http://www.scopus.com/inward/record.url?scp=78650967062&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2010.08.012
DO - 10.1016/j.jbiosc.2010.08.012
M3 - 文章
C2 - 20863755
AN - SCOPUS:78650967062
SN - 1389-1723
VL - 111
SP - 1
EP - 6
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 1
ER -
