TY - JOUR
T1 - Molecular cloning, characterization, and evolution analysis of the luciferase genes from three sympatric sibling fireflies (Lampyridae: Lampyrinae, Diaphanes)
AU - He, Jin Wu
AU - Liu, Gui Chun
AU - Dong, Ping Xuan
AU - Dong, Zhi Wei
AU - Zhao, Ruo Ping
AU - Wang, Wen
AU - Li, Xue Yan
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.
PY - 2021/8
Y1 - 2021/8
N2 - Firefly adult bioluminescence functions as signal communication between sexes. How sympatric sibling species with similar glow pattern recognize their conspecific mates remains largely unknown. To better understand the role of the luciferases of sympatric fireflies in recognizing mates, we cloned the luciferase genes of three sympatric forest dwelling fireflies (Diaphanes nubilus, Diaphanes pectinealis, and Diaphanes sp2) and evaluated their enzyme characteristics. Our data show that the amino acid (AA) sequences of all three luciferases are highly conserved, including the identities (D. nubilus vs D. pectinealis: 99%; D. nubilus vs Diaphanes sp2: 98.5%; D. pectinealis vs Diaphanes sp2: 99.4%) and the protein structures. Three recombinant luciferases produced in vitro all possess significant luminescence activity at pH 7.8, and similar maximum emission spectrum (D. nubilus: 562 nm; D. pectinealis and Diaphanes sp2: 564 nm). They show the highest activity at 10 °C (D. pectinealis, Diaphanes sp2) and 15 °C (D. nubilus), and completely inactivation at 45 °C. Their KM for d-luciferin and ATP were 2.7 μM and 92 μM (D. nubilus), 3.7 μM and 49 μM (D. pectinealis), 3.5 μM and 46 μM (Diaphanes sp2). Phylogenetic analyses support that D. nubilus is sister to D. pectinealis with Diaphanes sp2 at their base, which further cluster with Pyrocoelia. All combined data indicate that sympatric Diaphanes species have similar luciferase characteristics, suggesting that other strategies (e.g., pheromone, active time, etc.) may be adopted to recognize mates. Our data provide new insights into Diaphanes luciferases and their evolution. Graphic abstract: [Figure not available: see fulltext.]
AB - Firefly adult bioluminescence functions as signal communication between sexes. How sympatric sibling species with similar glow pattern recognize their conspecific mates remains largely unknown. To better understand the role of the luciferases of sympatric fireflies in recognizing mates, we cloned the luciferase genes of three sympatric forest dwelling fireflies (Diaphanes nubilus, Diaphanes pectinealis, and Diaphanes sp2) and evaluated their enzyme characteristics. Our data show that the amino acid (AA) sequences of all three luciferases are highly conserved, including the identities (D. nubilus vs D. pectinealis: 99%; D. nubilus vs Diaphanes sp2: 98.5%; D. pectinealis vs Diaphanes sp2: 99.4%) and the protein structures. Three recombinant luciferases produced in vitro all possess significant luminescence activity at pH 7.8, and similar maximum emission spectrum (D. nubilus: 562 nm; D. pectinealis and Diaphanes sp2: 564 nm). They show the highest activity at 10 °C (D. pectinealis, Diaphanes sp2) and 15 °C (D. nubilus), and completely inactivation at 45 °C. Their KM for d-luciferin and ATP were 2.7 μM and 92 μM (D. nubilus), 3.7 μM and 49 μM (D. pectinealis), 3.5 μM and 46 μM (Diaphanes sp2). Phylogenetic analyses support that D. nubilus is sister to D. pectinealis with Diaphanes sp2 at their base, which further cluster with Pyrocoelia. All combined data indicate that sympatric Diaphanes species have similar luciferase characteristics, suggesting that other strategies (e.g., pheromone, active time, etc.) may be adopted to recognize mates. Our data provide new insights into Diaphanes luciferases and their evolution. Graphic abstract: [Figure not available: see fulltext.]
UR - http://www.scopus.com/inward/record.url?scp=85111907780&partnerID=8YFLogxK
U2 - 10.1007/s43630-021-00080-4
DO - 10.1007/s43630-021-00080-4
M3 - 文章
C2 - 34347281
AN - SCOPUS:85111907780
SN - 1474-905X
VL - 20
SP - 1053
EP - 1067
JO - Photochemical and Photobiological Sciences
JF - Photochemical and Photobiological Sciences
IS - 8
ER -