Micron-sized flower-like Fe₃O₄@GMA magnetic porous microspheres for lipase immobilization

Flower-like Fe₃O₄ microspheres prepared by a fast solvothermal method were selected to fabricate micron-sized Fe₃O₄@glycidyl methacrylate (GMA) magnetic porous microspheres. The magnetic porous microspheres were endowed with appropriate specific surface area (39.54 m² g^-1) and appropriate pore diameter (16 nm), and could serve as a carrier for immobilized lipase. Meanwhile, the prepared magnetic microspheres with high saturation magnetization could realize rapid separations. After those microspheres were aminated and activated, the obtained immobilized lipase possessed high efficiency of immobilization and catalytic activity, with the optimum pH and temperature of 8.0 and 40 °C, respectively. The thermal stability of immobilized lipase was obviously exceeding free lipase.