Methods of studying mammalian cell migration and invasion in vitro

Peihong Su, Zhiping Miao, Lifang Hu, Runzhi Li, Chong Yin, Dijie Li, Zhihao Chen, Fan Zhao, Airong Qian

科研成果: 书/报告/会议事项章节会议稿件同行评审

2 引用 (Scopus)

摘要

Cell migration is a critical event for many physiological processes, such as embryonic development, renewal of skin and intestine, tissue repair, immune surveillance and so on. The abnormality of cell migration affects normal physiological processes and causes diseases, such as wound-healing delay of skin. Cell culture in vitro is essential for studying the migration process and the underlying mechanism. For cell migration detection in vitro, there are a number of methods and each has its own advantages and limitations. Transwell and wound healing assay are the most common methods used to study migration of different cells under different conditions. Besides, other assays are developed in the process of studying cell migration. In recently years, 3D cell migration attracts more and more attention for its accurate mimic of in vivo environment and it may be a trend of detecting cell migration and invasion in vitro in future. This paper summarizes current methods of studying cell migration in vitro and provides reference to researchers.

源语言英语
主期刊名Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
编辑Muhammad Zafar-uz-Zaman
出版商Institute of Electrical and Electronics Engineers Inc.
148-159
页数12
ISBN(电子版)9781467390736
DOI
出版状态已出版 - 1 3月 2017
活动14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017 - Islamabad, 巴基斯坦
期限: 10 1月 201714 1月 2017

出版系列

姓名Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017

会议

会议14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
国家/地区巴基斯坦
Islamabad
时期10/01/1714/01/17

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