Low affinity binding of plasma proteins to lipid-coated quantum dots as observed by in situ fluorescence correlation spectroscopy

Yvonne Klapper, Pauline Maffre, Li Shang, Kristina N. Ekdahl, Bo Nilsson, Simon Hettler, Manuel Dries, Dagmar Gerthsen, G. Ulrich Nienhaus

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29 引用 (Scopus)

摘要

Protein binding to lipid-coated nanoparticles has been pursued quantitatively by using fluorescence correlation spectroscopy. The binding of three important plasma proteins to lipid-enwrapped quantum dots (QDs) shows very low affinity, with an apparent dissociation coefficient in the range of several hundred micromolar. Thus, the tendency to adsorb is orders of magnitude weaker than for QDs coated with dihydrolipoic acid.

源语言英语
页(从-至)9980-9984
页数5
期刊Nanoscale
7
22
DOI
出版状态已出版 - 14 6月 2015
已对外发布

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