Function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome coronavirus

Zhinan Chen, Li Mi, Jing Xu, Jiyun Yu, Xianhui Wang, Jianli Jiang, Jinliang Xing, Peng Shang, Airong Qian, Yu Li, Peter X. Shaw, Jianwei Wang, Shumin Duan, Jin Ding, Chunmei Fan, Yang Zhang, Yong Yang, Xiaoling Yu, Qiang Feng, Biehu LiXiying Yao, Zheng Zhang, Ling Li, Xiaoping Xue, Ping Zhu

科研成果: 期刊稿件文章同行评审

229 引用 (Scopus)

摘要

To identify the function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome (SARS) coronavirus (CoV), we analyzed the protein-protein interaction among HAb18G/CD147, cyclophilin A (CyPA), and SARS-CoV structural proteins by coimmunoprecipitation and surface plasmon resonance analysis. Although none of the SARS-CoV proteins was found to be directly bound to HAb18G/CD147, the nucleocapsid (N) protein of SARS-CoV was bound to CyPA, which interacted with HAb18G/CD147. Further research showed that HAb18G/CD147, a transmembrane molecule, was highly expressed on 293 cells and that CyPA was integrated with SARS-CoV. HAb18G/CD147-antagonistic peptide (AP)-9, an AP of HAb18G/CD147, had a high rate of binding to 293 cells and an inhibitory effect on SARS-CoV. These results show that HAb18G/CD147, mediated by CyPA bound to SARS-CoV N protein, plays a functional role in facilitating invasion of host cells by SARS-CoV. Our findings provide some evidence for the cytologic mechanism of invasion by SARS-CoV and provide a molecular basis for screening anti-SARS drugs.

源语言英语
页(从-至)755-760
页数6
期刊Journal of Infectious Diseases
191
5
DOI
出版状态已出版 - 1 3月 2005
已对外发布

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