TY - JOUR
T1 - Conjugated Polymer Brush Based on Poly(l-lysine) with Efficient Ovalbumin Delivery for Dendritic Cell Vaccine
AU - Wang, Chao
AU - Sun, Pengfei
AU - Wang, Gaina
AU - Yuan, Pengcheng
AU - Jiang, Rongcui
AU - Wang, Wenjun
AU - Huang, Wei
AU - Fan, Quli
N1 - Publisher Copyright:
Copyright © 2018 American Chemical Society.
PY - 2018/12/17
Y1 - 2018/12/17
N2 - Dendritic cell (DC)-based vaccines consist of antigens and antigen-presenting cells, such as DCs, that can induce antitumor immune response and extend the lives of patients. In this research, a water-soluble conjugated polymer brush (WSCPB) made of poly(l-lysine) (PLL) and poly(p-phenyleneethynylene) (PPE) was applied to an antigen delivery system for the development of a DC vaccine. We synthesized the WSCPB with a lower proportion of the rigid PPE polymer backbone and a large amount of PLL side chains. The rigid backbone retained a stable optical performance within the experimental range, which enabled the visualization of the payload and cellular imaging as a reporter. Because of the unique brushlike structure, PPE-PLL exhibited not only excellent water solubility but also outstanding antigen-loading capacity. Ovalbumin (OVA), a model antigen in different research, could be adsorbed onto PPE-PLL and then taken up by DCs. Subsequently, DC maturation and cytokine release would be induced by the antigen. In vivo, strong immune responses were induced after the injection of antigen-pulsed DCs, and the level of cytokines in the serum was significantly increased. In addition, the study of the in vivo tumor-suppressor activity of these antigen-pulsed DCs revealed that the DC vaccine induced strong immune responses and thereby effectively inhibited tumor development.
AB - Dendritic cell (DC)-based vaccines consist of antigens and antigen-presenting cells, such as DCs, that can induce antitumor immune response and extend the lives of patients. In this research, a water-soluble conjugated polymer brush (WSCPB) made of poly(l-lysine) (PLL) and poly(p-phenyleneethynylene) (PPE) was applied to an antigen delivery system for the development of a DC vaccine. We synthesized the WSCPB with a lower proportion of the rigid PPE polymer backbone and a large amount of PLL side chains. The rigid backbone retained a stable optical performance within the experimental range, which enabled the visualization of the payload and cellular imaging as a reporter. Because of the unique brushlike structure, PPE-PLL exhibited not only excellent water solubility but also outstanding antigen-loading capacity. Ovalbumin (OVA), a model antigen in different research, could be adsorbed onto PPE-PLL and then taken up by DCs. Subsequently, DC maturation and cytokine release would be induced by the antigen. In vivo, strong immune responses were induced after the injection of antigen-pulsed DCs, and the level of cytokines in the serum was significantly increased. In addition, the study of the in vivo tumor-suppressor activity of these antigen-pulsed DCs revealed that the DC vaccine induced strong immune responses and thereby effectively inhibited tumor development.
KW - DC vaccine
KW - cancer immunotherapy
KW - poly(l-lysine)
KW - water-soluble conjugated polymer brush
UR - http://www.scopus.com/inward/record.url?scp=85072806096&partnerID=8YFLogxK
U2 - 10.1021/acsabm.8b00496
DO - 10.1021/acsabm.8b00496
M3 - 文章
C2 - 34996259
AN - SCOPUS:85072806096
SN - 2576-6422
VL - 1
SP - 1972
EP - 1982
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 6
ER -