TY - JOUR
T1 - Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells
AU - Chen, Xiaoyu
AU - Li, Rui
AU - Wong, Siu Hong Dexter
AU - Wei, Kongchang
AU - Cui, Miao
AU - Chen, Huaijun
AU - Jiang, Yuanzhang
AU - Yang, Boguang
AU - Zhao, Pengchao
AU - Xu, Jianbin
AU - Chen, Heng
AU - Yin, Chao
AU - Lin, Sien
AU - Lee, Wayne Yuk Wai
AU - Jing, Yihan
AU - Li, Zhen
AU - Yang, Zhengmeng
AU - Xia, Jiang
AU - Chen, Guosong
AU - Li, Gang
AU - Bian, Liming
N1 - Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Folded single chain polymeric nano-objects are the molecular level soft material with ultra-small size. Here, we report an easy and scalable method for preparing single-chain nanogels (SCNGs) with improved efficiency. We further investigate the impact of the dynamic molecular conformational change of SCNGs on cellular interactions from molecular to bulk scale. First, the supramolecular unfoldable SCNGs efficiently deliver siRNAs into stem cells as a molecular drug carrier in a conformation-dependent manner. Furthermore, the conformation changes of SCNGs enable dynamic and precise manipulation of ligand tether structure on 2D biomaterial interfaces to regulate the ligand–receptor ligation and mechanosensing of cells. Lastly, the dynamic SCNGs as the building blocks provide effective energy dissipation to bulk biomaterials such as hydrogels, thereby protecting the encapsulated stem cells from deleterious mechanical shocks in 3D matrix. Such a bottom-up molecular tailoring strategy will inspire further applications of single-chain nano-objects in the biomedical area.
AB - Folded single chain polymeric nano-objects are the molecular level soft material with ultra-small size. Here, we report an easy and scalable method for preparing single-chain nanogels (SCNGs) with improved efficiency. We further investigate the impact of the dynamic molecular conformational change of SCNGs on cellular interactions from molecular to bulk scale. First, the supramolecular unfoldable SCNGs efficiently deliver siRNAs into stem cells as a molecular drug carrier in a conformation-dependent manner. Furthermore, the conformation changes of SCNGs enable dynamic and precise manipulation of ligand tether structure on 2D biomaterial interfaces to regulate the ligand–receptor ligation and mechanosensing of cells. Lastly, the dynamic SCNGs as the building blocks provide effective energy dissipation to bulk biomaterials such as hydrogels, thereby protecting the encapsulated stem cells from deleterious mechanical shocks in 3D matrix. Such a bottom-up molecular tailoring strategy will inspire further applications of single-chain nano-objects in the biomedical area.
UR - http://www.scopus.com/inward/record.url?scp=85067612850&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-10640-z
DO - 10.1038/s41467-019-10640-z
M3 - 文章
C2 - 31221969
AN - SCOPUS:85067612850
SN - 2041-1723
VL - 10
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 2705
ER -