TY - JOUR
T1 - Compact photometric detector integrated with separation microchip for potential portable liquid chromatography system
AU - Jiao, Dezhao
AU - Zhang, Ruirong
AU - Wang, Mengbo
AU - Zhang, Xiaorui
AU - Ma, Haoquan
AU - Li, Mingyang
AU - Chang, Honglong
N1 - Publisher Copyright:
© 2024
PY - 2024/8/30
Y1 - 2024/8/30
N2 - In recent years, miniaturized analytical instruments have been developing to meet the needs of portable and rapid analysis. The key of miniaturized analytical equipment is the miniaturization and integration of functional modules. This paper aims to develop a miniaturized photometric detector and separation microfluidic chip for a liquid chromatography (LC) system. The detector uses a light-emitting diode to emit ultraviolet light, which is collimated by an internal double lens. A Z-shaped flow cell with a long optical path is designed and fabricated in the separation microfluidic chip with a three-layer structure, which provides a tubing-free connection between the separation and detection unit. Detector performance is evaluated using hemoglobin (Hb) samples, with an upper limit of detection linearity (95 %) of 0.345 AU and stray light level as low as 0.08 %. Additionally, the microchip channel can be filled with cation exchange resin and C18 particles. Finally, an ion LC system and a reversed-phase LC system were constructed based on the miniaturized photometric detector and two microchips with different packed columns, respectively, and were successfully used in the separation and detection of two metabolic markers (glycated hemoglobin or bilirubin). The results of this study are expected to facilitate the development of a portable LC system and their application in community health services and family health management of chronic diseases.
AB - In recent years, miniaturized analytical instruments have been developing to meet the needs of portable and rapid analysis. The key of miniaturized analytical equipment is the miniaturization and integration of functional modules. This paper aims to develop a miniaturized photometric detector and separation microfluidic chip for a liquid chromatography (LC) system. The detector uses a light-emitting diode to emit ultraviolet light, which is collimated by an internal double lens. A Z-shaped flow cell with a long optical path is designed and fabricated in the separation microfluidic chip with a three-layer structure, which provides a tubing-free connection between the separation and detection unit. Detector performance is evaluated using hemoglobin (Hb) samples, with an upper limit of detection linearity (95 %) of 0.345 AU and stray light level as low as 0.08 %. Additionally, the microchip channel can be filled with cation exchange resin and C18 particles. Finally, an ion LC system and a reversed-phase LC system were constructed based on the miniaturized photometric detector and two microchips with different packed columns, respectively, and were successfully used in the separation and detection of two metabolic markers (glycated hemoglobin or bilirubin). The results of this study are expected to facilitate the development of a portable LC system and their application in community health services and family health management of chronic diseases.
KW - Liquid chromatography
KW - Microfluidic chip
KW - Photometric detector
KW - Stray light
KW - Z-shaped cell
UR - http://www.scopus.com/inward/record.url?scp=85198995615&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2024.465175
DO - 10.1016/j.chroma.2024.465175
M3 - 文章
C2 - 39032217
AN - SCOPUS:85198995615
SN - 0021-9673
VL - 1731
JO - Journal of Chromatography A
JF - Journal of Chromatography A
M1 - 465175
ER -