Bio-panning of antagonistic peptides against HAb18G/CD147 and their function of anti-hepatoma invasion

Bao cheng Huang, Peng Shang, Ai rong Qian, Xian hui Wang, Guang hua Shi, Zhi nan Chen

科研成果: 期刊稿件文章同行评审

3 引用 (Scopus)

摘要

OBJECTIVE: To screen out the HAb18G/CD147 binding peptides and find out an antagonist against hepatoma invasion. METHODS: HAb18G/CD147 was purified by affinity chromatographic method and the antigen binding peptides acquired by bio-panning a phage-displayed 12-peptide library. After obtaining the sequence of the selected phage-displayed peptides, all the 9 peptides were synthesized by solid-phase method and identified by mass spectrograph. The peptides' anti-metastatic function was tested by Boyden Chamber assay. RESULTS: The purified HAb18G/CD147, identified by Western blot (molecular weight about 65 kd) could be used to bio-pan the phage-displayed peptide library. After 3 rounds of bio-panning, 9 positive phage clones were selected and sequenced. The synthesized peptides had uneven inhibitory activities and three of them were able to markedly inhibit the hepatoma cell invasion (P < 0.01). The most effective peptide decreased by 90.1% of hepatoma cells migrating through the Boyden Chamber membrane as compared with the control. CONCLUSION: Bio-panning the phage-displayed peptide library can be used successfully to screen out the antigen binding peptides. Hepatoma metastatic potential can be inhibited by peptide antagonist which could be a good foundation of developing peptide therapeutic agent against hepatoma metastasis.

源语言英语
页(从-至)111-114
页数4
期刊Zhonghua zhong liu za zhi [Chinese journal of oncology]
25
2
出版状态已出版 - 3月 2003

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