TY - JOUR
T1 - Anti-tumor potential of cell free culture supernatant of Lactobacillus rhamnosus strains isolated from human breast milk
AU - Riaz Rajoka, Muhammad Shahid
AU - Zhao, Haobin
AU - Mehwish, Hafiza Mahreen
AU - Li, Na
AU - Lu, Yao
AU - Lian, Ziyang
AU - Shao, Dongyan
AU - Jin, Mingliang
AU - Li, Qi
AU - Zhao, Liqing
AU - Shi, Junling
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/9
Y1 - 2019/9
N2 - Lactobacilli rhamnosus has been characterized as a probiotic and plays an important role in human health by stimulating the supplement of nutrients, preventing the colonization of pathogens, and influencing the immune system. This study investigated the anticancer activity of the three Lactobacillus rhamnosus strains SHA111, SHA112, and SHA113 isolated from human breast milk. The cell-free supernatant of a liquid culture of the three strains showed excellent antioxidant activities against DPPH free radicals, superoxide anion radicals, and hydroxyl radicals; furthermore, significant anticancer activity was found on cervix cancer cells (HeLa)via cytotoxicity and induction of apoptosis. RT-qPCR and western blot analysis showed the induction of apoptosis was achieved via the up-regulation of BAD, BAX, Caspase3, Caspase8, Caspase9, and down-regulation of BCL-2 genes in HeLa cells. The results suggest that these strains have potential anticancer capability.
AB - Lactobacilli rhamnosus has been characterized as a probiotic and plays an important role in human health by stimulating the supplement of nutrients, preventing the colonization of pathogens, and influencing the immune system. This study investigated the anticancer activity of the three Lactobacillus rhamnosus strains SHA111, SHA112, and SHA113 isolated from human breast milk. The cell-free supernatant of a liquid culture of the three strains showed excellent antioxidant activities against DPPH free radicals, superoxide anion radicals, and hydroxyl radicals; furthermore, significant anticancer activity was found on cervix cancer cells (HeLa)via cytotoxicity and induction of apoptosis. RT-qPCR and western blot analysis showed the induction of apoptosis was achieved via the up-regulation of BAD, BAX, Caspase3, Caspase8, Caspase9, and down-regulation of BCL-2 genes in HeLa cells. The results suggest that these strains have potential anticancer capability.
KW - Antioxidant
KW - Antitumor
KW - Apoptosis
KW - Lactobacillus
KW - Probiotics
UR - http://www.scopus.com/inward/record.url?scp=85065236714&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2019.05.002
DO - 10.1016/j.foodres.2019.05.002
M3 - 文章
C2 - 31284979
AN - SCOPUS:85065236714
SN - 0963-9969
VL - 123
SP - 286
EP - 297
JO - Food Research International
JF - Food Research International
ER -
