A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes

Xingwei Chen; Jiajia Xu; Fengtai Suo; Changmin Yu; Duoteng Zhang; Jian Chen; Qiong Wu; Su Jing; Lin Li; Wei Huang

doi:10.1016/j.saa.2019.117949

A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes

Xingwei Chen, Jiajia Xu, Fengtai Suo, Changmin Yu, Duoteng Zhang, Jian Chen, Qiong Wu, Su Jing, Lin Li, Wei Huang

柔性电子研究院

科研成果: 期刊稿件 › 文章 › 同行评审

12 引用（Scopus）

摘要

The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn²⁺. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn²⁺ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn²⁺ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn²⁺ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn²⁺ in the living cells and zebrafishes.

源语言	英语
文章编号	117949
期刊	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
卷	229
DOI	https://doi.org/10.1016/j.saa.2019.117949
出版状态	已出版 - 15 3月 2020

访问文件

10.1016/j.saa.2019.117949

其它文件与链接

链接到 Scopus 的出版物

引用此

Chen, X., Xu, J., Suo, F., Yu, C., Zhang, D., Chen, J., Wu, Q., Jing, S., Li, L., & Huang, W. (2020). A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 229, 文章 117949. https://doi.org/10.1016/j.saa.2019.117949

@article{334d4e59bdc645b68de38f62a39db8b5,

title = "A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes",

abstract = "The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn2+. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn2+ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn2+ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn2+ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn2+ in the living cells and zebrafishes.",

keywords = "High-throughput, Imaging, Paper-based devices, Point-of-care testing, Zinc ions",

author = "Xingwei Chen and Jiajia Xu and Fengtai Suo and Changmin Yu and Duoteng Zhang and Jian Chen and Qiong Wu and Su Jing and Lin Li and Wei Huang",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2020",

month = mar,

day = "15",

doi = "10.1016/j.saa.2019.117949",

language = "英语",

volume = "229",

journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",

issn = "1386-1425",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes

AU - Chen, Xingwei

AU - Xu, Jiajia

AU - Suo, Fengtai

AU - Yu, Changmin

AU - Zhang, Duoteng

AU - Chen, Jian

AU - Wu, Qiong

AU - Jing, Su

AU - Li, Lin

AU - Huang, Wei

PY - 2020/3/15

Y1 - 2020/3/15

N2 - The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn2+. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn2+ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn2+ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn2+ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn2+ in the living cells and zebrafishes.

AB - The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn2+. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn2+ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn2+ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn2+ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn2+ in the living cells and zebrafishes.

KW - High-throughput

KW - Imaging

KW - Paper-based devices

KW - Point-of-care testing

KW - Zinc ions

UR - http://www.scopus.com/inward/record.url?scp=85076474666&partnerID=8YFLogxK

U2 - 10.1016/j.saa.2019.117949

DO - 10.1016/j.saa.2019.117949

M3 - 文章

C2 - 31864152

AN - SCOPUS:85076474666

SN - 1386-1425

VL - 229

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

M1 - 117949

ER -

A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes

摘要

访问文件

其它文件与链接

指纹

引用此