A new method to realize high-throughput protein crystallization in a superconducting magnet

Lin Jun Huang; Hui Ling Cao; Ya Jing Ye; Yong Ming Liu; Chen Yan Zhang; Qin Qin Lu; Hai Hou; Peng Shang; Da Chuan Yin

doi:10.1039/c4ce01892c

A new method to realize high-throughput protein crystallization in a superconducting magnet

Lin Jun Huang, Hui Ling Cao, Ya Jing Ye, Yong Ming Liu, Chen Yan Zhang, Qin Qin Lu, Hai Hou, Peng Shang, Da Chuan Yin

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9 引用（Scopus）

摘要

We present a new method for the realization of high-throughput protein crystallization screening using an array of 96 capillaries aligned in a circle. In this method, each capillary represents a single crystallization condition, and all capillaries experience identical magnetic field conditions. After crystallization, the crystals in the capillary can be directly diffracted without harvesting. This method proved easy to perform and is applicable for use in magnetic fields and may be further extended for use in other circumstances, for example, under space microgravity conditions.

源语言	英语
页（从-至）	1237-1241
页数	5
期刊	CrystEngComm
卷	17
期	6
DOI	https://doi.org/10.1039/c4ce01892c
出版状态	已出版 - 14 2月 2015

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abstract = "We present a new method for the realization of high-throughput protein crystallization screening using an array of 96 capillaries aligned in a circle. In this method, each capillary represents a single crystallization condition, and all capillaries experience identical magnetic field conditions. After crystallization, the crystals in the capillary can be directly diffracted without harvesting. This method proved easy to perform and is applicable for use in magnetic fields and may be further extended for use in other circumstances, for example, under space microgravity conditions.",

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AU - Huang, Lin Jun

AU - Cao, Hui Ling

AU - Ye, Ya Jing

AU - Liu, Yong Ming

AU - Zhang, Chen Yan

AU - Lu, Qin Qin

AU - Hou, Hai

AU - Shang, Peng

AU - Yin, Da Chuan

PY - 2015/2/14

Y1 - 2015/2/14

N2 - We present a new method for the realization of high-throughput protein crystallization screening using an array of 96 capillaries aligned in a circle. In this method, each capillary represents a single crystallization condition, and all capillaries experience identical magnetic field conditions. After crystallization, the crystals in the capillary can be directly diffracted without harvesting. This method proved easy to perform and is applicable for use in magnetic fields and may be further extended for use in other circumstances, for example, under space microgravity conditions.

AB - We present a new method for the realization of high-throughput protein crystallization screening using an array of 96 capillaries aligned in a circle. In this method, each capillary represents a single crystallization condition, and all capillaries experience identical magnetic field conditions. After crystallization, the crystals in the capillary can be directly diffracted without harvesting. This method proved easy to perform and is applicable for use in magnetic fields and may be further extended for use in other circumstances, for example, under space microgravity conditions.

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DO - 10.1039/c4ce01892c

M3 - 文章

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SP - 1237

EP - 1241

JO - CrystEngComm

JF - CrystEngComm

IS - 6

ER -

A new method to realize high-throughput protein crystallization in a superconducting magnet

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