TY - JOUR
T1 - Whole body radioprotective activity of an acetone-water extract from the seedpod of Nelumbo nucifera Gaertn. seedpod
AU - Duan, Y.
AU - Zhang, H.
AU - Xie, B.
AU - Yan, Y.
AU - Li, J.
AU - Xu, F.
AU - Qin, Y.
PY - 2010/12
Y1 - 2010/12
N2 - Procyanidins extracted with acetone-water from lotus (Nelumbo nucifera Gaertn.) seedpod (LSPCs) were evaluated for in vivo radioprotective activity against whole body gamma irradiation in Swiss albino mice. Pretreated with LSPCs 200mg/kg by intragastric (i.g.) for 15days was found to be the most effective dose in preventing radiation sickness, reducing radiation-induced mortality, increasing mean survival time and elevating radiation median lethal dose (LD50) from 8.9 to 10.5Gy, indicating a dose modifying factor (DMF) of 1.18. Further, administered LSPCs at a dose of 200mg/kg could effectively maintain spleen index close to normal, stimulate endogenous spleen colony forming units, promote the levels of red blood cells (RBC), white blood cells (WBC), platelets and hemoglobin in peripheral blood, and prevent spleen and skin damage in irradiated mice, reduce the level of radiation-induced micronucleated polychromatic erythrocytes in bone marrow, maintain the polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) ratio (P/N ratio) and significantly decrease bone marrow chromosomal damage. Alternatively, pretreated with LSPCs (200mg/kg) significantly decreased the lipid peroxidation (LPO) level, and elevated the activities of endogenous antioxidant enzymes in liver after irradiation. Thus LSPCs possess a strong whole body radioprotective activity, and it may be used as a radioprotector.
AB - Procyanidins extracted with acetone-water from lotus (Nelumbo nucifera Gaertn.) seedpod (LSPCs) were evaluated for in vivo radioprotective activity against whole body gamma irradiation in Swiss albino mice. Pretreated with LSPCs 200mg/kg by intragastric (i.g.) for 15days was found to be the most effective dose in preventing radiation sickness, reducing radiation-induced mortality, increasing mean survival time and elevating radiation median lethal dose (LD50) from 8.9 to 10.5Gy, indicating a dose modifying factor (DMF) of 1.18. Further, administered LSPCs at a dose of 200mg/kg could effectively maintain spleen index close to normal, stimulate endogenous spleen colony forming units, promote the levels of red blood cells (RBC), white blood cells (WBC), platelets and hemoglobin in peripheral blood, and prevent spleen and skin damage in irradiated mice, reduce the level of radiation-induced micronucleated polychromatic erythrocytes in bone marrow, maintain the polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) ratio (P/N ratio) and significantly decrease bone marrow chromosomal damage. Alternatively, pretreated with LSPCs (200mg/kg) significantly decreased the lipid peroxidation (LPO) level, and elevated the activities of endogenous antioxidant enzymes in liver after irradiation. Thus LSPCs possess a strong whole body radioprotective activity, and it may be used as a radioprotector.
KW - Irradiation injury
KW - Lotus seedpod
KW - Procyanidins
KW - Radioprotective activity
UR - http://www.scopus.com/inward/record.url?scp=78049448799&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2010.09.008
DO - 10.1016/j.fct.2010.09.008
M3 - 文章
C2 - 20837084
AN - SCOPUS:78049448799
SN - 0278-6915
VL - 48
SP - 3374
EP - 3384
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 12
ER -