Tryptophan supplementation and pH adjustment for optimizing the sporulation of Coniothyrium minitans

Junling Shi, Yin Li, Yi Zheng, Yi Zhu, Xiaoping Zhang, Guocheng Du, Jian Chen

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

To determine the effect of tryptophan and pH on sporulation of Coniothyrium minitans, the fungus was cultivated using a two-stage, agar plate method in which addition of tryptophan and pH were controlled at the sporulation stage. The spore yield was enhanced by 4 times with 0.1 g tryptophan/l addition after 72 h. The optimal pH values were 4 for mycelia growth and 5.8-6 for sporulation. Mycelia grown at pH 6 had a higher productivity of spore production than did those grown at pH 4.

Original languageEnglish
Pages (from-to)259-262
Number of pages4
JournalBiotechnology Letters
Volume30
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

Keywords

  • Cellulose acetate membrane
  • Coniothyrium minitans
  • Tryptophan

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