TY - JOUR
T1 - Synthesis of paramagnetic dendritic silica nanomaterials with fibrous pore structure (Fe3O4@KCC-1) and their application in immobilization of lipase from
T2 - Candida rugosa with enhanced catalytic activity and stability
AU - Ali, Zafar
AU - Tian, Lei
AU - Zhang, Baoliang
AU - Ali, Nisar
AU - Khan, Muhammad
AU - Zhang, Qiuyu
N1 - Publisher Copyright:
© 2017 The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.
PY - 2017
Y1 - 2017
N2 - Paramagnetic mesoporous fibrous silica (Fe3O4@KCC-1) was prepared and its surface was functionalized with 3-aminopropyltriethoxysilane (APTES) to obtain amino functionalized magnetic nanoparticles. Lipase from Candida rugosa was immobilized on functionalized magnetite Fe3O4@KCC-1-NH2 using glutaraldehyde (GLU) as the linker. The nanoparticles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), powder X-ray diffraction (XRD), vibrating sample magnetometry (VSM) and Fourier transformed infrared spectroscopy (FTIR). The characterization results revealed successful immobilization of lipase on functionalized magnetite with a saturation magnetization of 62.73 and 42.65 emu g-1 for magnetic nanoparticles and carbonyl functionalized carriers, respectively. The applied approach for support preparation, activation, and optimization of immobilization conditions, with a good lipase loading of 283 mg g-1 of carrier, provided better resistance to temperature and pH inactivation than free lipase and hence expanded the reaction pH and temperature regions, with an optimum pH of 6 and temperature of 35 °C. Immobilized Lipase Candida rugosa (ICRL) showed enzyme activity of 630 U g-1, maintained above 560.7 U g-1 (89%) of the initial activity after 28 days and 434.7 U g-1 (69%) after 10 cycles. Thus, ICRL showed improved storage stability and reusability.
AB - Paramagnetic mesoporous fibrous silica (Fe3O4@KCC-1) was prepared and its surface was functionalized with 3-aminopropyltriethoxysilane (APTES) to obtain amino functionalized magnetic nanoparticles. Lipase from Candida rugosa was immobilized on functionalized magnetite Fe3O4@KCC-1-NH2 using glutaraldehyde (GLU) as the linker. The nanoparticles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), powder X-ray diffraction (XRD), vibrating sample magnetometry (VSM) and Fourier transformed infrared spectroscopy (FTIR). The characterization results revealed successful immobilization of lipase on functionalized magnetite with a saturation magnetization of 62.73 and 42.65 emu g-1 for magnetic nanoparticles and carbonyl functionalized carriers, respectively. The applied approach for support preparation, activation, and optimization of immobilization conditions, with a good lipase loading of 283 mg g-1 of carrier, provided better resistance to temperature and pH inactivation than free lipase and hence expanded the reaction pH and temperature regions, with an optimum pH of 6 and temperature of 35 °C. Immobilized Lipase Candida rugosa (ICRL) showed enzyme activity of 630 U g-1, maintained above 560.7 U g-1 (89%) of the initial activity after 28 days and 434.7 U g-1 (69%) after 10 cycles. Thus, ICRL showed improved storage stability and reusability.
UR - http://www.scopus.com/inward/record.url?scp=85027168556&partnerID=8YFLogxK
U2 - 10.1039/c7nj01912b
DO - 10.1039/c7nj01912b
M3 - 文章
AN - SCOPUS:85027168556
SN - 1144-0546
VL - 41
SP - 8222
EP - 8231
JO - New Journal of Chemistry
JF - New Journal of Chemistry
IS - 16
ER -