TY - JOUR
T1 - Surfactant-assisted preparation of nanohybrid for simultaneously improving enzyme-immobilization and electron-transfer in biosensor and biofuel cell
AU - Qu, Fengjin
AU - Ma, Xiaoyan
AU - Hui, Yuchen
AU - Chen, Fang
AU - Gao, Yan
AU - Chen, Ying
N1 - Publisher Copyright:
© 2017, Springer-Verlag Berlin Heidelberg.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - As enzyme-immobilization and electron-transfer are the key factors for fabricating an enzymatic bioelectrode and its devices, we investigated a strategy to simultaneously improve the two aspects by assistance of the cationic surfactant, stearyltrimethylammonium bromide (STAB). By electrodeposition method, we obtained a multifunctional STAB-modified nanomaterial on electrode surface to improve enzyme-immobilization and electron-transfer. On the one hand, STAB could firmly adsorb a substantial number of enzymes via electrostatic interaction in a favorable orientation on the conductive nanomaterial surface for electron-transfer. On the other hand, STAB acts as a dispersant and stabilizer for traditionally conductive nanomaterials (reduced graphene oxide, carbon nanotubes, and gold nanoparticles) to guarantee their unique properties and form a well-conductive network. Electrochemical measurements demonstrated that enzymatic electrodes based on the nanohybrid possessed fast electron-transfer rate, a large quantity of immobilized enzymes, and good activity toward glucose oxidation or oxygen reduction. The glucose biosensor performed linear response range of 0.01–11.71 mM, detection limit of 3.84 × 10−3 mM, and sensitivity of 10.42 μA mM−1 cm−2, while the glucose/O2 biofuel cell exhibited maximum power density of 121.87 μW cm−2 and open-circuit voltage of 0.663 V. Both of the devices showed better performances than those of devices without STAB or conductive nanomaterials in this work. [Figure not available: see fulltext.]
AB - As enzyme-immobilization and electron-transfer are the key factors for fabricating an enzymatic bioelectrode and its devices, we investigated a strategy to simultaneously improve the two aspects by assistance of the cationic surfactant, stearyltrimethylammonium bromide (STAB). By electrodeposition method, we obtained a multifunctional STAB-modified nanomaterial on electrode surface to improve enzyme-immobilization and electron-transfer. On the one hand, STAB could firmly adsorb a substantial number of enzymes via electrostatic interaction in a favorable orientation on the conductive nanomaterial surface for electron-transfer. On the other hand, STAB acts as a dispersant and stabilizer for traditionally conductive nanomaterials (reduced graphene oxide, carbon nanotubes, and gold nanoparticles) to guarantee their unique properties and form a well-conductive network. Electrochemical measurements demonstrated that enzymatic electrodes based on the nanohybrid possessed fast electron-transfer rate, a large quantity of immobilized enzymes, and good activity toward glucose oxidation or oxygen reduction. The glucose biosensor performed linear response range of 0.01–11.71 mM, detection limit of 3.84 × 10−3 mM, and sensitivity of 10.42 μA mM−1 cm−2, while the glucose/O2 biofuel cell exhibited maximum power density of 121.87 μW cm−2 and open-circuit voltage of 0.663 V. Both of the devices showed better performances than those of devices without STAB or conductive nanomaterials in this work. [Figure not available: see fulltext.]
KW - Biofuel cell
KW - Biosensor
KW - Cationic surfactant
KW - Electrostatic interaction
KW - Enzyme-immobilization
UR - http://www.scopus.com/inward/record.url?scp=85011263771&partnerID=8YFLogxK
U2 - 10.1007/s10008-017-3509-3
DO - 10.1007/s10008-017-3509-3
M3 - 文章
AN - SCOPUS:85011263771
SN - 1432-8488
VL - 21
SP - 1545
EP - 1557
JO - Journal of Solid State Electrochemistry
JF - Journal of Solid State Electrochemistry
IS - 6
ER -