Surface treatment by oxidizing the plates can alter the response of protein crystallization

Yun Zhu Guo; Yong Ming Liu; Da Chuan Yin; Jin He; Chen Yan Zhang; Hui Ling Cao; Hai Hou; Wei Ma; Wei Hong Guo; Jian Yu Shi; Chao Cui; Zhe Wang; Peng Shang

doi:10.1107/S1600576713030999

Surface treatment by oxidizing the plates can alter the response of protein crystallization

Yun Zhu Guo, Yong Ming Liu, Da Chuan Yin, Jin He, Chen Yan Zhang, Hui Ling Cao, Hai Hou, Wei Ma, Wei Hong Guo, Jian Yu Shi, Chao Cui, Zhe Wang, Peng Shang

School of life sciences

Northwestern Polytechnical University Xian

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

This report describes the modification of crystallization plates by simply oxidizing the surface of the protein wells. The oxidized crystallization plates were tested in standard protein crystallization screening and reproducibility studies. The results showed that the protein wells of the treated plates were smoother and more optically transparent than those of the untreated plates, and more importantly, protein crystallization was significantly promoted after the oxidation treatment. Because there is no change to the routine screening protocol, this method is simple and easy to apply in protein crystallization.

Original language	English
Pages (from-to)	228-236
Number of pages	9
Journal	Journal of Applied Crystallography
Volume	47
Issue number	1
DOIs	https://doi.org/10.1107/S1600576713030999
State	Published - Feb 2014

Keywords

crystallization plates
crystallization screening
oxidation
protein crystallization
reproducibility

Access to Document

10.1107/S1600576713030999

Cite this

@article{0302a8f8cd504e179c0bf46913b49157,

title = "Surface treatment by oxidizing the plates can alter the response of protein crystallization",

abstract = "This report describes the modification of crystallization plates by simply oxidizing the surface of the protein wells. The oxidized crystallization plates were tested in standard protein crystallization screening and reproducibility studies. The results showed that the protein wells of the treated plates were smoother and more optically transparent than those of the untreated plates, and more importantly, protein crystallization was significantly promoted after the oxidation treatment. Because there is no change to the routine screening protocol, this method is simple and easy to apply in protein crystallization.",

keywords = "crystallization plates, crystallization screening, oxidation, protein crystallization, reproducibility",

author = "Guo, {Yun Zhu} and Liu, {Yong Ming} and Yin, {Da Chuan} and Jin He and Zhang, {Chen Yan} and Cao, {Hui Ling} and Hai Hou and Wei Ma and Guo, {Wei Hong} and Shi, {Jian Yu} and Chao Cui and Zhe Wang and Peng Shang",

year = "2014",

month = feb,

doi = "10.1107/S1600576713030999",

language = "英语",

volume = "47",

pages = "228--236",

journal = "Journal of Applied Crystallography",

issn = "0021-8898",

publisher = "International Union of Crystallography",

number = "1",

}

TY - JOUR

T1 - Surface treatment by oxidizing the plates can alter the response of protein crystallization

AU - Guo, Yun Zhu

AU - Liu, Yong Ming

AU - Yin, Da Chuan

AU - He, Jin

AU - Zhang, Chen Yan

AU - Cao, Hui Ling

AU - Hou, Hai

AU - Ma, Wei

AU - Guo, Wei Hong

AU - Shi, Jian Yu

AU - Cui, Chao

AU - Wang, Zhe

AU - Shang, Peng

PY - 2014/2

Y1 - 2014/2

N2 - This report describes the modification of crystallization plates by simply oxidizing the surface of the protein wells. The oxidized crystallization plates were tested in standard protein crystallization screening and reproducibility studies. The results showed that the protein wells of the treated plates were smoother and more optically transparent than those of the untreated plates, and more importantly, protein crystallization was significantly promoted after the oxidation treatment. Because there is no change to the routine screening protocol, this method is simple and easy to apply in protein crystallization.

AB - This report describes the modification of crystallization plates by simply oxidizing the surface of the protein wells. The oxidized crystallization plates were tested in standard protein crystallization screening and reproducibility studies. The results showed that the protein wells of the treated plates were smoother and more optically transparent than those of the untreated plates, and more importantly, protein crystallization was significantly promoted after the oxidation treatment. Because there is no change to the routine screening protocol, this method is simple and easy to apply in protein crystallization.

KW - crystallization plates

KW - crystallization screening

KW - oxidation

KW - protein crystallization

KW - reproducibility

UR - http://www.scopus.com/inward/record.url?scp=84893340784&partnerID=8YFLogxK

U2 - 10.1107/S1600576713030999

DO - 10.1107/S1600576713030999

M3 - 文章

AN - SCOPUS:84893340784

SN - 0021-8898

VL - 47

SP - 228

EP - 236

JO - Journal of Applied Crystallography

JF - Journal of Applied Crystallography

IS - 1

ER -

Surface treatment by oxidizing the plates can alter the response of protein crystallization

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this