Study on the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells

Ai Rong Qian; Peng Shang; Yu Li; Chun Mei Fan; Dan Chen; Chun Mei Wang; Ping Zhu; Zhi Nan Chen

Study on the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells

Ai Rong Qian, Peng Shang, Yu Li, Chun Mei Fan, Dan Chen, Chun Mei Wang, Ping Zhu, Zhi Nan Chen

Air Force Medical University

Research output: Contribution to journal › Article › peer-review

Abstract

AIM: To study the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells.

METHODS: Fluorescence-activated cell sorting analysis(FACS) was used to detect the expression of HAb18G antigen on HHCC and SMMC7721 cells. Antagonistic peptides(APs) AP-1, AP-2 and AP-6 were labelled with biotin at their N-terminals. The binding abilities of APs to HHCC or SMMC7721 cells were determined by FACS and confocal microscope.

RESULTS: HAb18G antigen was highly expressed on the HHCC and SMMC7721 cells. AP-6 has the highest affinity for HHCC and SMMC7721 cells compared with AP-1 and AP-2.

CONCLUSION: Antagonistic peptides of HAb18G/CD147 have high affinities with HAb18G/CD147 on the hepatocellular carcinoma cells.

Original language	English
Pages (from-to)	127-129
Number of pages	3
Journal	Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Volume	19
Issue number	2
State	Published - 1 Mar 2003
Externally published	Yes

Cite this

@article{98fbd763260247e4b621f961012ad1fd,

title = "Study on the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells",

abstract = "AIM: To study the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells.METHODS: Fluorescence-activated cell sorting analysis(FACS) was used to detect the expression of HAb18G antigen on HHCC and SMMC7721 cells. Antagonistic peptides(APs) AP-1, AP-2 and AP-6 were labelled with biotin at their N-terminals. The binding abilities of APs to HHCC or SMMC7721 cells were determined by FACS and confocal microscope.RESULTS: HAb18G antigen was highly expressed on the HHCC and SMMC7721 cells. AP-6 has the highest affinity for HHCC and SMMC7721 cells compared with AP-1 and AP-2.CONCLUSION: Antagonistic peptides of HAb18G/CD147 have high affinities with HAb18G/CD147 on the hepatocellular carcinoma cells.",

author = "Qian, {Ai Rong} and Peng Shang and Yu Li and Fan, {Chun Mei} and Dan Chen and Wang, {Chun Mei} and Ping Zhu and Chen, {Zhi Nan}",

year = "2003",

month = mar,

day = "1",

language = "英语",

volume = "19",

pages = "127--129",

journal = "Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology",

issn = "1007-8738",

publisher = "Fourth Military Medical University",

number = "2",

}

TY - JOUR

T1 - Study on the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells

AU - Qian, Ai Rong

AU - Shang, Peng

AU - Li, Yu

AU - Fan, Chun Mei

AU - Chen, Dan

AU - Wang, Chun Mei

AU - Zhu, Ping

AU - Chen, Zhi Nan

PY - 2003/3/1

Y1 - 2003/3/1

N2 - AIM: To study the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells.METHODS: Fluorescence-activated cell sorting analysis(FACS) was used to detect the expression of HAb18G antigen on HHCC and SMMC7721 cells. Antagonistic peptides(APs) AP-1, AP-2 and AP-6 were labelled with biotin at their N-terminals. The binding abilities of APs to HHCC or SMMC7721 cells were determined by FACS and confocal microscope.RESULTS: HAb18G antigen was highly expressed on the HHCC and SMMC7721 cells. AP-6 has the highest affinity for HHCC and SMMC7721 cells compared with AP-1 and AP-2.CONCLUSION: Antagonistic peptides of HAb18G/CD147 have high affinities with HAb18G/CD147 on the hepatocellular carcinoma cells.

AB - AIM: To study the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells.METHODS: Fluorescence-activated cell sorting analysis(FACS) was used to detect the expression of HAb18G antigen on HHCC and SMMC7721 cells. Antagonistic peptides(APs) AP-1, AP-2 and AP-6 were labelled with biotin at their N-terminals. The binding abilities of APs to HHCC or SMMC7721 cells were determined by FACS and confocal microscope.RESULTS: HAb18G antigen was highly expressed on the HHCC and SMMC7721 cells. AP-6 has the highest affinity for HHCC and SMMC7721 cells compared with AP-1 and AP-2.CONCLUSION: Antagonistic peptides of HAb18G/CD147 have high affinities with HAb18G/CD147 on the hepatocellular carcinoma cells.

UR - http://www.scopus.com/inward/record.url?scp=84983050265&partnerID=8YFLogxK

M3 - 文章

C2 - 15151746

AN - SCOPUS:84983050265

SN - 1007-8738

VL - 19

SP - 127

EP - 129

JO - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology

JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology

IS - 2

ER -

Study on the affinities of antagonistic peptides with HAb18G/CD147 on the hepatocellular carcinoma cells

Abstract

Other files and links

Fingerprint

Cite this