siRNA targeted against HAb18G/CD147 inhibits MMP-2 secretion, actin and FAK expression in hepatocellular carcinoma cell line via ERK1/2 pathway

Hui Yun Xu; Ai Rong Qian; Peng Shang; Jing Xu; Ling Min Kong; Hui Jie Bian; Zhi Nan Chen

doi:10.1016/j.canlet.2006.05.017

siRNA targeted against HAb18G/CD147 inhibits MMP-2 secretion, actin and FAK expression in hepatocellular carcinoma cell line via ERK1/2 pathway

Hui Yun Xu, Ai Rong Qian, Peng Shang, Jing Xu, Ling Min Kong, Hui Jie Bian, Zhi Nan Chen

School of life sciences

Cell Engineering Research Centre

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

HAb18G/CD147 has been identified as a factor that induces MMPs production. SiRNA targeted against HAb18G/CD147 was transfected into FHCC-98 cells (a HCC cell line) to knockdown its expression. The results showed that downregulating HAb18G/CD147 decreased ERK1/2, MMP-2 and FAK levels and inhibited cell motility and invasion, together with rearranged actin stress fiber formation, while had no effects on integrin α3β1 expression. MEK1/2 inhibitor, U0126, inhibited MMP-2, FAK and actin expression in FHCC-98 cell line. The findings indicate that si-HAb18G inhibits gelatinase production, actin and FAK expression in FHCC-98 via an ERK1/2 signaling pathway.

Original language	English
Pages (from-to)	336-344
Number of pages	9
Journal	Cancer Letters
Volume	247
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2006.05.017
State	Published - 18 Mar 2007

Keywords

ERK1/2
FAK
HAb18G/CD147
MMP-2
Microfilament
Tumor metastasis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2006.05.017

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title = "siRNA targeted against HAb18G/CD147 inhibits MMP-2 secretion, actin and FAK expression in hepatocellular carcinoma cell line via ERK1/2 pathway",

abstract = "HAb18G/CD147 has been identified as a factor that induces MMPs production. SiRNA targeted against HAb18G/CD147 was transfected into FHCC-98 cells (a HCC cell line) to knockdown its expression. The results showed that downregulating HAb18G/CD147 decreased ERK1/2, MMP-2 and FAK levels and inhibited cell motility and invasion, together with rearranged actin stress fiber formation, while had no effects on integrin α3β1 expression. MEK1/2 inhibitor, U0126, inhibited MMP-2, FAK and actin expression in FHCC-98 cell line. The findings indicate that si-HAb18G inhibits gelatinase production, actin and FAK expression in FHCC-98 via an ERK1/2 signaling pathway.",

keywords = "ERK1/2, FAK, HAb18G/CD147, MMP-2, Microfilament, Tumor metastasis",

author = "Xu, {Hui Yun} and Qian, {Ai Rong} and Peng Shang and Jing Xu and Kong, {Ling Min} and Bian, {Hui Jie} and Chen, {Zhi Nan}",

year = "2007",

month = mar,

day = "18",

doi = "10.1016/j.canlet.2006.05.017",

language = "英语",

volume = "247",

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journal = "Cancer Letters",

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T1 - siRNA targeted against HAb18G/CD147 inhibits MMP-2 secretion, actin and FAK expression in hepatocellular carcinoma cell line via ERK1/2 pathway

AU - Xu, Hui Yun

AU - Qian, Ai Rong

AU - Shang, Peng

AU - Xu, Jing

AU - Kong, Ling Min

AU - Bian, Hui Jie

AU - Chen, Zhi Nan

PY - 2007/3/18

Y1 - 2007/3/18

N2 - HAb18G/CD147 has been identified as a factor that induces MMPs production. SiRNA targeted against HAb18G/CD147 was transfected into FHCC-98 cells (a HCC cell line) to knockdown its expression. The results showed that downregulating HAb18G/CD147 decreased ERK1/2, MMP-2 and FAK levels and inhibited cell motility and invasion, together with rearranged actin stress fiber formation, while had no effects on integrin α3β1 expression. MEK1/2 inhibitor, U0126, inhibited MMP-2, FAK and actin expression in FHCC-98 cell line. The findings indicate that si-HAb18G inhibits gelatinase production, actin and FAK expression in FHCC-98 via an ERK1/2 signaling pathway.

AB - HAb18G/CD147 has been identified as a factor that induces MMPs production. SiRNA targeted against HAb18G/CD147 was transfected into FHCC-98 cells (a HCC cell line) to knockdown its expression. The results showed that downregulating HAb18G/CD147 decreased ERK1/2, MMP-2 and FAK levels and inhibited cell motility and invasion, together with rearranged actin stress fiber formation, while had no effects on integrin α3β1 expression. MEK1/2 inhibitor, U0126, inhibited MMP-2, FAK and actin expression in FHCC-98 cell line. The findings indicate that si-HAb18G inhibits gelatinase production, actin and FAK expression in FHCC-98 via an ERK1/2 signaling pathway.

KW - ERK1/2

KW - FAK

KW - HAb18G/CD147

KW - MMP-2

KW - Microfilament

KW - Tumor metastasis

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U2 - 10.1016/j.canlet.2006.05.017

DO - 10.1016/j.canlet.2006.05.017

M3 - 文章

C2 - 16815629

AN - SCOPUS:33846904601

SN - 0304-3835

VL - 247

SP - 336

EP - 344

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

siRNA targeted against HAb18G/CD147 inhibits MMP-2 secretion, actin and FAK expression in hepatocellular carcinoma cell line via ERK1/2 pathway

Abstract

Keywords

UN SDGs

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