TY - JOUR
T1 - Single-Molecule Analysis of MicroRNA and Logic Operations Using a Smart Plasmonic Nanobiosensor
AU - Zhang, Ying
AU - Shuai, Zhenhua
AU - Zhou, Hao
AU - Luo, Zhimin
AU - Liu, Bing
AU - Zhang, Yinan
AU - Zhang, Lei
AU - Chen, Shufen
AU - Chao, Jie
AU - Weng, Lixing
AU - Fan, Quli
AU - Fan, Chunhai
AU - Huang, Wei
AU - Wang, Lianhui
N1 - Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/3/21
Y1 - 2018/3/21
N2 - Analysis of biomolecules at the single-molecule level is a great challenge in molecular diagnostics, gene profiling, and environmental monitoring. In this work, we design a smart plasmonic nanobiosensor based on individual Au@Ag core-shell nanocube (Au@Ag NC) modified with tetrahedron-structured DNA (tsDNA) for detecting microRNA 21 (miR-21) at the single-molecule level. An average localized surface plasmon resonance (LSPR) scattering spectral wavelength shift of approximately 0.4 nm can be obtained for a single miR-21 hybridization event on the nanobiosensor. In addition, the sensing mechanism of the individual Au@Ag NC is further verified by the three-dimensional finite-difference time-domain (3D-FDTD) simulations. Notably, this system not only allows the real-time detection of miR-21 with an aM level sensitivity over a large dynamic range from 1 aM to 1 nM, but also enables DNA-based logic operations as well as biomemory by exploiting miR-21, KpnI, and StuI-responsive assays. Our study opens a unique method for single-molecule detection of biomolecules and thus holds great promise in a variety of biological and biomedical applications.
AB - Analysis of biomolecules at the single-molecule level is a great challenge in molecular diagnostics, gene profiling, and environmental monitoring. In this work, we design a smart plasmonic nanobiosensor based on individual Au@Ag core-shell nanocube (Au@Ag NC) modified with tetrahedron-structured DNA (tsDNA) for detecting microRNA 21 (miR-21) at the single-molecule level. An average localized surface plasmon resonance (LSPR) scattering spectral wavelength shift of approximately 0.4 nm can be obtained for a single miR-21 hybridization event on the nanobiosensor. In addition, the sensing mechanism of the individual Au@Ag NC is further verified by the three-dimensional finite-difference time-domain (3D-FDTD) simulations. Notably, this system not only allows the real-time detection of miR-21 with an aM level sensitivity over a large dynamic range from 1 aM to 1 nM, but also enables DNA-based logic operations as well as biomemory by exploiting miR-21, KpnI, and StuI-responsive assays. Our study opens a unique method for single-molecule detection of biomolecules and thus holds great promise in a variety of biological and biomedical applications.
UR - http://www.scopus.com/inward/record.url?scp=85044233430&partnerID=8YFLogxK
U2 - 10.1021/jacs.7b12772
DO - 10.1021/jacs.7b12772
M3 - 文章
C2 - 29504757
AN - SCOPUS:85044233430
SN - 0002-7863
VL - 140
SP - 3988
EP - 3993
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 11
ER -