Scalable Focusing in Single-Cell Analysis Enabled by Amplitude Modulated Positive Dielectrophoresis

Zuyuan Tian, Shaoxi Wang, Xihua Wang, Jie Chen

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In conventional stream-based single-cell analysis, hydrodynamical focusing methods typically require constraining sample flow or complex channel structures, which can adversely impact analysis throughput and is unsuitable for low-concentration samples. In this study, we introduced a microfluidic device that employed the positive dielectrophoresis (DEP) force to focus cells, eliminating the need for flow manipulation or clog-sensitive structures that compromise the system throughput. To mitigate the potential issues of cell attachment induced by strong attracting force, we employed a squarewave to modulate the amplitude of the DEP signal, enabling efficient cell focusing with amplitude-modulated positive DEP force (AM-pDEP). The theoretical model is constructed to facilitate parameter optimization and demonstrate its suitability for low-concentration samples. Additionally, focusing performance is verified with a side-arranged Coulter counter to form an impedance-based on-chip flow cytometer with scalable analysis throughput.

Original languageEnglish
Title of host publication2024 IEEE Biomedical Circuits and Systems Conference, BioCAS 2024
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9798350354959
DOIs
StatePublished - 2024
Event2024 IEEE Biomedical Circuits and Systems Conference, BioCAS 2024 - Xi�an, China
Duration: 24 Oct 202426 Oct 2024

Publication series

Name2024 IEEE Biomedical Circuits and Systems Conference, BioCAS 2024

Conference

Conference2024 IEEE Biomedical Circuits and Systems Conference, BioCAS 2024
Country/TerritoryChina
CityXi�an
Period24/10/2426/10/24

Keywords

  • Dielectrophoresis
  • microfluidics
  • particle focusing
  • single-cell analysis

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