Real-time monitoring of the solution concentration variation during the crystallization process of protein-lysozyme by using digital holographic interferometry

Yanyan Zhang, Jianlin Zhao, Jianglei Di, Hongzhen Jiang, Qian Wang, Jun Wang, Yunzhu Guo, Dachuan Yin

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

We report a real-time measurement method of the solution concentration variation during the growth of protein-lysozyme crystals based on digital holographic interferometry. A series of holograms containing the information of the solution concentration variation in the whole crystallization process is recorded by CCD. Based on the principle of double-exposure holographic interferometry and the relationship between the phase difference of the reconstructed object wave and the solution concentration, the solution concentration variation with time for arbitrary point in the solution can be obtained, and then the two-dimensional concentration distribution of the solution during crystallization process can also be figured out under the precondition which the refractive index is constant through the light propagation direction. The experimental results turns out that it is feasible to in situ, full-field and real-time monitor the crystal growth process by using this method.

Original languageEnglish
Pages (from-to)18415-18421
Number of pages7
JournalOptics Express
Volume20
Issue number16
DOIs
StatePublished - 30 Jul 2012

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