Real-time and wide-field mapping of cell-substrate adhesion gap and its evolution via surface plasmon resonance holographic microscopy

Siqing Dai, Tongyao Yu, Jiwei Zhang, Hua Lu, Jiazhen Dou, Mengmeng Zhang, Chen Dong, Jianglei Di, Jianlin Zhao

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

As one of the most common biological phenomena, cell adhesion plays a vital role in the cellular activities such as the growth and apoptosis, attracting tremendous research interests over the past decades. Taking the cell evolution under drug injection as an example, the dynamics of cell-substrate adhesion gap can provide valuable information in the fundamental research of cell contacts. A robust technique of monitoring the cell adhesion gap and its evolution in real time is highly desired. Herein, we develop a surface plasmon resonance holographic microscopy to achieve the novel functionality of real-time and wide-field mapping of the cell-substrate adhesion gap and its evolution in situ. The cell adhesion gap images of mouse osteoblast cells and human breast cancer cells have been effectively extracted in a dynamic and label-free manner. The proposed technique opens up a new avenue of revealing the cell-substrate interaction mechanism and renders the wide applications in the biosensing area.

Original languageEnglish
Article number112826
JournalBiosensors and Bioelectronics
Volume174
DOIs
StatePublished - 15 Feb 2021

Keywords

  • Bottom membrane dynamics
  • Cell-substrate adhesion gap
  • Digital holographic microscopy
  • Living cell sensing
  • Surface plasmon resonance

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