TY - JOUR
T1 - Preparation, characterization and antioxidant activity of polysaccharide from spent Lentinus edodes substrate
AU - Zhu, Hongji
AU - Tian, Li
AU - Zhang, Lei
AU - Bi, Jingxiu
AU - Song, Qianqian
AU - Yang, Hui
AU - Qiao, Jianjun
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/6
Y1 - 2018/6
N2 - This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide. The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated. Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity. The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051 mg/mL, 0.379 mg/mL, 0.719 mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F). After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77 kDa. Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition. FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups. The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
AB - This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide. The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated. Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity. The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051 mg/mL, 0.379 mg/mL, 0.719 mg/mL, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F). After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77 kDa. Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition. FT-IR and NMR spectroscopy analysis revealed that PSP has both α and β glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked α-D-Manp residue with some acetyl groups. The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate.
KW - Antioxidant activity
KW - Drying procedures
KW - Polysaccharide
KW - Spent Lentinus edodes substrate
UR - http://www.scopus.com/inward/record.url?scp=85042210885&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2018.01.196
DO - 10.1016/j.ijbiomac.2018.01.196
M3 - 文章
C2 - 29428383
AN - SCOPUS:85042210885
SN - 0141-8130
VL - 112
SP - 976
EP - 984
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -
