Portable liquid chromatography for point-of-care testing of glycated haemoglobin

Ding Li; Haiyan Chen; Shuang Ren; Yanfang Zhang; Yang Yang; Honglong Chang

doi:10.1016/j.snb.2019.127484

Portable liquid chromatography for point-of-care testing of glycated haemoglobin

Ding Li, Haiyan Chen, Shuang Ren, Yanfang Zhang, Yang Yang, Honglong Chang

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Liquid chromatography (LC) coupled with absorption detection plays an important role in the analytical field. However, the miniaturisation of the whole system is still a challenging problem. Here we developed a compact and lightweight LC system with overall size of 20 cm × 16 cm × 16 cm and weight of 1.8 kg for the fast and accurate measurement of glycated haemoglobin (HbA_1c). The system mainly comprises a battery-operated electrolysis micropump (EMP), a highly integrated LC microchip and an LED-based absorbance detector. The good linearity (R² = 0.9860) and low limit of detection (LOD) (about nine times lower than the commercial detector) of the LED detector were measured to meet the requirement of the on-chip determination. The inaccuracy (≤ 4.3 %) and repeatability (≤ 2.8 %) of the LC system were successfully verified by measuring the HbA_1c levels of patients with diabetes and comparing the results with those from a commercial LC instrument. Thus, the developed LC system showed great potential for accurate and point-of-care diabetes diagnostics.

Original language	English
Article number	127484
Journal	Sensors and Actuators, B: Chemical
Volume	305
DOIs	https://doi.org/10.1016/j.snb.2019.127484
State	Published - 15 Feb 2020

Keywords

Diabetes diagnosis
Glycated haemoglobin
Liquid chromatography
Point-of-care testing
Portable

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2019.127484

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title = "Portable liquid chromatography for point-of-care testing of glycated haemoglobin",

abstract = "Liquid chromatography (LC) coupled with absorption detection plays an important role in the analytical field. However, the miniaturisation of the whole system is still a challenging problem. Here we developed a compact and lightweight LC system with overall size of 20 cm × 16 cm × 16 cm and weight of 1.8 kg for the fast and accurate measurement of glycated haemoglobin (HbA1c). The system mainly comprises a battery-operated electrolysis micropump (EMP), a highly integrated LC microchip and an LED-based absorbance detector. The good linearity (R2 = 0.9860) and low limit of detection (LOD) (about nine times lower than the commercial detector) of the LED detector were measured to meet the requirement of the on-chip determination. The inaccuracy (≤ 4.3 %) and repeatability (≤ 2.8 %) of the LC system were successfully verified by measuring the HbA1c levels of patients with diabetes and comparing the results with those from a commercial LC instrument. Thus, the developed LC system showed great potential for accurate and point-of-care diabetes diagnostics.",

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author = "Ding Li and Haiyan Chen and Shuang Ren and Yanfang Zhang and Yang Yang and Honglong Chang",

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AU - Li, Ding

AU - Chen, Haiyan

AU - Ren, Shuang

AU - Zhang, Yanfang

AU - Yang, Yang

AU - Chang, Honglong

PY - 2020/2/15

Y1 - 2020/2/15

N2 - Liquid chromatography (LC) coupled with absorption detection plays an important role in the analytical field. However, the miniaturisation of the whole system is still a challenging problem. Here we developed a compact and lightweight LC system with overall size of 20 cm × 16 cm × 16 cm and weight of 1.8 kg for the fast and accurate measurement of glycated haemoglobin (HbA1c). The system mainly comprises a battery-operated electrolysis micropump (EMP), a highly integrated LC microchip and an LED-based absorbance detector. The good linearity (R2 = 0.9860) and low limit of detection (LOD) (about nine times lower than the commercial detector) of the LED detector were measured to meet the requirement of the on-chip determination. The inaccuracy (≤ 4.3 %) and repeatability (≤ 2.8 %) of the LC system were successfully verified by measuring the HbA1c levels of patients with diabetes and comparing the results with those from a commercial LC instrument. Thus, the developed LC system showed great potential for accurate and point-of-care diabetes diagnostics.

AB - Liquid chromatography (LC) coupled with absorption detection plays an important role in the analytical field. However, the miniaturisation of the whole system is still a challenging problem. Here we developed a compact and lightweight LC system with overall size of 20 cm × 16 cm × 16 cm and weight of 1.8 kg for the fast and accurate measurement of glycated haemoglobin (HbA1c). The system mainly comprises a battery-operated electrolysis micropump (EMP), a highly integrated LC microchip and an LED-based absorbance detector. The good linearity (R2 = 0.9860) and low limit of detection (LOD) (about nine times lower than the commercial detector) of the LED detector were measured to meet the requirement of the on-chip determination. The inaccuracy (≤ 4.3 %) and repeatability (≤ 2.8 %) of the LC system were successfully verified by measuring the HbA1c levels of patients with diabetes and comparing the results with those from a commercial LC instrument. Thus, the developed LC system showed great potential for accurate and point-of-care diabetes diagnostics.

KW - Diabetes diagnosis

KW - Glycated haemoglobin

KW - Liquid chromatography

KW - Point-of-care testing

KW - Portable

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ER -

Portable liquid chromatography for point-of-care testing of glycated haemoglobin

Abstract

Keywords

UN SDGs

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