Polyvinylamine and Its Derivative as Effective Carrier for Targeted Delivery of Small RNAs

Ye Tian, Suryaji Patil, Xudong Deng, Yipu Zhao, Bo Sang, Airong Qian

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations

Abstract

Polymeric delivery systems could enable the fast- and low-side-effect transport of various RNA classes. Previously, we demonstrated that polyvinylamine (PVAm), a cationic polymer, transfects many kinds of RNAs with high efficiency and low toxicity both in vitro and in vivo. The modification of poly lactic-co-glycolic acid (PLGA) with cartilage-targeting peptide (CAP) enhances its stiffness and tissue-specific delivery of RNA to overcome the avascular nature of articular cartilage. Here we describe the protocol to use PVAm as an RNA carrier, and further, by modifying PVAm with PLGA and CAP, the corresponding co-polymer could be applied for functional RNA delivery for osteoarthritis treatment.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages353-365
Number of pages13
DOIs
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2822
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • CAP-PVAm-PLGA
  • Cartilage-targeting
  • In vitro and in vivo
  • PVAm
  • RNA delivery system

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